ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRSFTQSIED EGPPELPASL DIDMIEGVSM KTSDTNDTII EVKPGKVDPQ LGETQKYKMA 

        70         80         90        100        110        120 
VPGPGHSEQE QELHKSLIRD EEEDAEVEQV FAASAEPVVT PPKSLNFAET AEELRHAYDQ 

       130        140        150        160        170        180 
KIRKRPTTPT SACVLDGIAF FAGSVDGSND TIPTDAEKKI KVSIPSGRRR KTENMSYSEF 

       190        200        210        220        230        240 
YESMANQIPG GGGRRMSQSG RTTPLHDYEG DILDENPQYK DKAPPAPVVH RRSSIEWENF 

       250        260        270        280        290        300 
ADMEDKMEQA EEKARKEEKK EKEETAEVAQ NDDVTTEKHQ NEVEKSKKML NFLAKPPTVE 

       310        320        330        340        350        360 
ITSPDAPHQG AFHDNTPKEP KVVEEEEDDD LPTFSEDMDE ARQQVMKEMT ESVVKTENVE 

       370        380        390        400        410        420 
NEKQEDTHIS EGHVEYGTEE SPAVTDGSYQ QDGTENAYDP NAYPGYIWNY ETQEWEYDPS 

       430        440        450        460        470        480 
YVAPEEPIDQ SAYEAQAAAY AEVDQRAAEV VYQREHAGYD QGDFYTHQDT SYSSEAYAHD 

       490        500        510        520        530        540 
QAKGYQDPNA YGAEAAADNS QEYDYSAYGG YEGYLAACRA YDEESQQPQY GETVATEAYS 

       550        560        570        580        590        600 
QDYGYEQQGD YHHEGVGATD ENSTSAISGY EQNGASTSLG YGDAQGYDGY DHNASYYQGQ 

       610        620        630        640        650        660 
EYQQEYYQNH KYENSQYQVD QHPAESAAPP RPVAPMEPLF KQAPPQPDPF GWDSSGHTGD 

       670        680        690        700        710        720 
APTASEPVQE LSPTPEASSS TGTSAPARPP PARPEAPKKD ETAVDAAAPP RPPPAARPPP 

       730        740        750        760        770        780 
PRPAPANKEK PKEPEPEEDA WAQFKRMTEK VSTAVKSTES TLKNLEETSA ANDIKDESYL 

       790        800        810        820        830        840 
ANVGGSQGFV NESTQKEIQR LTEEKKMEKL QKKKLKQQGK KAATPTLEPD EEDAMDRAAQ 

       850        860        870        880        890        900 
ELAMKMASMR SDMADWKAPE MIPVKEIKKA PEIRRVDSAS AIPPRKRSSI KDVQQDSGGS 

       910        920        930        940        950        960 
LELPPHLAAP QDSIAPNPKG DHAPDDPILS APAWADFETS EPMLPPSESG FFSNKDASNE 

       970        980        990       1000       1010       1020 
GGVSRDATDD PFVTTVASSE KRSSFVADPF APQQAALIDE SYDPFAVVAV EEVVAMAKAK 

      1030       1040       1050       1060       1070       1080 
AEQAAANAEN EDDFYNGRQS PTLSTPTPEG GSPISQQRPN AFEDDFKCAE LTGLDTPTPL 

      1090       1100       1110       1120       1130       1140 
YDEDDSQPLT DFIPKFDGDG WDLMVRHPIK KKSFMAERCW KPCYVRLHGL TLYLYNDKKD 

      1150       1160       1170       1180       1190       1200 
AQPIQELLLQ ATYSLSDTTL QAYDVYGKIH TVKLQFVVYK EKVGIRPGQI SRLVDGHITK 

      1210       1220       1230       1240       1250       1260 
YGLPLEHSAQ CTVLLKFGSL NASHLQTFVT TVEDLLFKCK ITRTAKPVYK QDEVQIHCYD 

      1270       1280       1290       1300       1310       1320 
EYSAFVDKEG ILSDQKARVR LFCLAFLTGS PVLEVGLNDR RRQGKEIVRR KDILPMYTER 

      1330       1340       1350       1360       1370       1380 
WIRFEALEFH SIVNKPEFDK EQVIAFSPPD GCFFEIMRFR VRPPRNREKA MTVKSIMKIA 

      1390       1400       1410       1420       1430       1440 
GSKVEIRIEA MAAAQIQRTR GSDERRNIPC EDIAIRFPIP EAWIYLFREE RHWGVGSIHS 

      1450       1460       1470       1480       1490       1500 
KKLRPGKVKN LKDRLLGAVQ ASEPNLIECA IGEAKYEHVY RSLVWRIPRL PEKHHAAYKS 

      1510       1520       1530       1540       1550       1560 
HLLKCRFELS SFDLMPEEFL PRCDVDFTMP LATVSNTVVR SVSVEQHEDS DRVEKFVRYV 

      1570       1580       1590       1600       1610       1620 
AKYQYKVEID YVQCADLDLD MADPSVNPEA AAAPVPELHQ PTFNPSTQES DTQQGYRIDF 

      1630       1640       1650 
NEAEMGGSNR RDDSSSDEEP DSHKMPIIQI DMKNYGY 

References and documentation are available.

Number of amino acids: 1657

Molecular weight: 185705.37

Theoretical pI: 4.78

Amino acid composition: 
Ala (A) 156	  9.4%
Arg (R)  77	  4.6%
Asn (N)  49	  3.0%
Asp (D) 124	  7.5%
Cys (C)  15	  0.9%
Gln (Q)  90	  5.4%
Glu (E) 170	 10.3%
Gly (G)  84	  5.1%
His (H)  38	  2.3%
Ile (I)  65	  3.9%
Leu (L)  81	  4.9%
Lys (K) 108	  6.5%
Met (M)  37	  2.2%
Phe (F)  51	  3.1%
Pro (P) 131	  7.9%
Ser (S) 120	  7.2%
Thr (T)  84	  5.1%
Trp (W)  13	  0.8%
Tyr (Y)  72	  4.3%
Val (V)  92	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 294
Total number of positively charged residues (Arg + Lys): 185

Atomic composition:

Carbon      C	      8133
Hydrogen    H	     12528
Nitrogen    N	      2224
Oxygen      O	      2661
Sulfur      S	        52

Formula: C8133H12528N2224O2661S52
Total number of atoms: 25598

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   179655
Abs 0.1% (=1 g/l)   0.967, assuming all pairs of Cys residues form cystines


Ext. coefficient   178780
Abs 0.1% (=1 g/l)   0.963, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.75
This classifies the protein as unstable.



Aliphatic index: 59.88

Grand average of hydropathicity (GRAVY): -0.839