ProtParam
User-provided sequence:
10 20 30 40 50 60
MRSHHRHFSS YVSFILFLFL FFISFSSSTS KLEPAKSEPK RKHSVSAILV FGDSTVDPGN
70 80 90 100 110 120
NNYIDTVFKC NFPPYGLDFR NKTPTGRFCN GRLVTDFIAS YIGVKENVPP YLDPNLGINE
130 140 150 160 170 180
LISGVSFASA GSGYDPLTPT ITNVIDIPTQ LEYFREYKRK LEGKMGKQEM EKHIEEAMFC
190 200 210 220 230 240
VSAGTNDFVI NYFTIPIRRK TFTIEAYQQF VISNLKQFIQ GLWKEGARKI TVAGLPPIGC
250 260 270 280 290 300
LPIVITLFSG EALTNRRCID RFSTVATNYN FLLQKQLALM QVGLAHLGSK IFYLDVYNPV
310 320 330 340 350 360
YEVIRDPRKF GFEEVFSGCC GSGYLEASFL CNPKSYVCPN TSAYVFFDSI HPSEKTYFSL
370
FRSLRPIYDS ILGSF
References and
documentation are available.
Number of amino acids: 375
Molecular weight: 42481.84
Theoretical pI: 8.71
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1951
Hydrogen H 2967
Nitrogen N 493
Oxygen O 544
Sulfur S 14
Formula: C
1951H
2967N
493O
544S
14
Total number of atoms: 5969
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34310
Abs 0.1% (=1 g/l) 0.808, assuming all pairs of Cys residues form cystines
Ext. coefficient 33810
Abs 0.1% (=1 g/l) 0.796, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.63
This classifies the protein as stable.
Aliphatic index: 83.41
Grand average of hydropathicity (GRAVY): -0.040