ProtParam
User-provided sequence:
10 20 30 40 50 60
MRSIKCVIIG DGAVGKTSLL ISYTTNSFPT DYVPTVFDNY STTIAIPNGT ASSPLELDNG
70 80 90 100 110 120
NDKRGSLSSA SSSPSTDRKL YKINLWDTAG QEDYDRLRPL CYPQTDIFLI CFSVSEHASF
130 140 150 160 170 180
ANVTEKWLPE LKQTSNIEGT SLYTKLGKYP ILLVGTKSDL RDDPATQKKL QEANSDYVSQ
190 200 210 220 230 240
EEIDELVQRC GFMGYTECSA ATQAGVREVF EQAVRYAIYE PESPNQKSAN HTLTDELTTA
250 260 270 280 290 300
TTNTNGDKNI REQKQQPHHN NSTDSTLPKG SLQQEKEALN IKPTKKGQKD KIHEQSKSKG
310 320 330
SKIASNNHHN KQAKPKTRND KKKKKSKCVI L
References and
documentation are available.
Number of amino acids: 331
Molecular weight: 36818.15
Theoretical pI: 8.68
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1600
Hydrogen H 2563
Nitrogen N 453
Oxygen O 526
Sulfur S 8
Formula: C
1600H
2563N
453O
526S
8
Total number of atoms: 5150
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29255
Abs 0.1% (=1 g/l) 0.795, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.784, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.61
This classifies the protein as unstable.
Aliphatic index: 67.79
Grand average of hydropathicity (GRAVY): -0.831