ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRSKHVLYIA ILFSSIFGGK GIQQNEEFQR YDGWYNNLAN SEWGSAGSRL HRDARSYYSD 

        70         80         90        100        110        120 
GVYSVNNSLP SARELSDILF KGESGIPNTR GCTTLLAFFS QVVAYEIMQS NGVSCPLETL 

       130        140        150        160        170        180 
KIQVPLCDNV FDKECEGKTE IPFTRAKYDK ATGNGLNSPR EQINERTSWI DGSFIYGTTQ 

       190        200        210        220        230        240 
PWVSSLRSFK QGRLAEGVPG YPPLNNPHIP LNNPAPPQVH RLMSPDRLFM LGDSRVNENP 

       250        260        270        280        290        300 
GLLSFGLILF RWHNYNANQI HREHPDWTDE QIFQAARRLV IASMQKIIAY DFVPGLLGED 

       310        320        330        340        350        360 
VRLSNYTKYM PHVPPGISHA FGAAAFRFPH SIVPPAMLLR KRGNKCEFRT EVGGYPALRL 

       370        380        390        400        410        420 
CQNWWNAQDI VKEYSVDEII LGMASQIAER DDNIVVEDLR DYIFGPMHFS RLDVVASSIM 

       430        440        450        460        470        480 
RGRDNGVPPY NELRRTFGLA PKTWETMNED FYKKHTAKVE KLKELYGGNI LYLDAYVGGM 

       490        500        510        520        530        540 
LEGGENGPGE LFKEIIKDQF TRIRDGDRFW FENKLNGLFT DEEVQMIHSI TLRDIIKATT 

       550        560        570        580        590        600 
DIDETMLQKD VFFFKEGDPC PQPFQVNTTG LEPCVPFMQS TYWTDNDTTY VFTLIGLACV 

       610        620        630        640        650        660 
PLICYGIGRY LVNRRIAIGH NSACDSLTTD FANDDCGAKG DIYGVNALEW LQEEYIRQVR 

       670        680        690        700        710        720 
IEIENTTLAV KKPRGGILRK IRFETGQKIE LFHSMPNPSA MHGPFVLLSQ KNNHHLVIRL 

       730        740        750        760        770        780 
SSDRDLSKFL DQIRQAASGI NAEVIIKDEE NSILLSQAIT KERRQDRLDL FFREAYAKAF 

       790        800        810        820        830        840 
NDSELQDSET SFDSSNDDIL NETISREELA SAMGMKANNE FVKRMFAMTA KHNEDSLSFN 

       850        860        870        880        890        900 
EFLTVLREFV NAPQKQKLQT LFKMCDLEGK NKVLRKDLAE LVKSLNQTAG VHITESVQLR 

       910        920        930        940        950        960 
LFNEVLHYAG VSNDAKYLTY DDFNALFSDI PDKQPVGLPF NRKNYQPSIG ETSSLNSFAV 

       970        980        990       1000       1010       1020 
VDRSINSSAP LTLIHKVSAF LETYRQHVFI VFCFVAINLV LFFERFWHYR YMAENRDLRR 

      1030       1040       1050       1060       1070       1080 
VMGAGIAITR GAAGALSFCM ALILLTVCRN IITLLRETVI AQYIPFDSAI AFHKIVALFA 

      1090       1100       1110       1120       1130       1140 
AFWATLHTVG HCVNFYHVGT QSQEGLACLF QEAFFGSNFL PSISYWFFST ITGLTGIALV 

      1150       1160       1170       1180       1190       1200 
AVMCIIYVFA LPCFIKRAYH AFRLTHLLNI AFYALTLLHG LPKLLDSPKF GYYVVGPIVL 

      1210       1220       1230       1240       1250       1260 
FVIDRIIGLM QYYKKLEIVN AEILPSDIIY IEYRRPREFK YKSGQWVTVS SPSISCTFNE 

      1270       1280       1290       1300       1310       1320 
SHAFSIASSP QDENMKLYIK AVGPWTWKLR SELIRSLNTG SPFPLIHMKG PYGDGNQEWM 

      1330       1340       1350       1360       1370       1380 
DYEVAIMVGA GIGVTPYAST LVDLVQRTSS DSFHRVRCRK VYFLWVCSTH KNYEWFVDVL 

      1390       1400       1410       1420       1430       1440 
KNVEDQARSG ILETHIFVTQ TFHKFDLRTT MLYICEKHFR ATNSGISMFT GLHAKNHFGR 

      1450       1460       1470       1480       1490 
PNFKAFFQFI QSEHKEQSKI GVFSCGPVNL NESIAEGCAD ANRQRDAPSF AHRFETF 

References and documentation are available.

Number of amino acids: 1497

Molecular weight: 170415.81

Theoretical pI: 6.76

Amino acid composition: 
Ala (A)  99	  6.6%
Arg (R)  86	  5.7%
Asn (N)  81	  5.4%
Asp (D)  75	  5.0%
Cys (C)  26	  1.7%
Gln (Q)  55	  3.7%
Glu (E)  88	  5.9%
Gly (G)  94	  6.3%
His (H)  42	  2.8%
Ile (I) 104	  6.9%
Leu (L) 135	  9.0%
Lys (K)  71	  4.7%
Met (M)  33	  2.2%
Phe (F) 100	  6.7%
Pro (P)  65	  4.3%
Ser (S) 102	  6.8%
Thr (T)  75	  5.0%
Trp (W)  21	  1.4%
Tyr (Y)  56	  3.7%
Val (V)  89	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 163
Total number of positively charged residues (Arg + Lys): 157

Atomic composition:

Carbon      C	      7706
Hydrogen    H	     11835
Nitrogen    N	      2067
Oxygen      O	      2193
Sulfur      S	        59

Formula: C7706H11835N2067O2193S59
Total number of atoms: 23860

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   200565
Abs 0.1% (=1 g/l)   1.177, assuming all pairs of Cys residues form cystines


Ext. coefficient   198940
Abs 0.1% (=1 g/l)   1.167, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.20
This classifies the protein as unstable.



Aliphatic index: 86.12

Grand average of hydropathicity (GRAVY): -0.181