ProtParam
User-provided sequence:
10 20 30 40 50 60
MRSLNTLLLS LFVAMSSGAP LLKIREEKNS SLPSIKIFGT GGTIASKGST SATTAGYSVG
70 80 90 100 110 120
LTVNDLIEAV PSLAEKANLD YLQVSNVGSN SLNYTHLIPL YHGISEALAS DDYAGAVVTH
130 140 150 160 170 180
GTDTMEETAF FLDLTINSEK PVCIAGAMRP ATATSADGPM NLYQAVSIAA SEKSLGRGTM
190 200 210 220 230 240
ITLNDRIASG FWTTKMNANS LDTFRADEQG YLGYFSNDDV EFYYPPVKPN GWQFFDISNL
250 260 270 280 290 300
TDPSEIPEVI ILYSYQGLNP ELIVKAVKDL GAKGIVLAGS GAGSWTATGS IVNEQLYEEY
310 320 330 340 350 360
GIPIVHSRRT ADGTVPPDDA PEYAIGSGYL NPQKSRILLQ LCLYSGYGMD QIRSVFSGVY
GG
References and
documentation are available.
Number of amino acids: 362
Molecular weight: 38686.58
Theoretical pI: 4.69
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1726
Hydrogen H 2689
Nitrogen N 443
Oxygen O 545
Sulfur S 10
Formula: C
1726H
2689N
443O
545S
10
Total number of atoms: 5413
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44935
Abs 0.1% (=1 g/l) 1.162, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 1.158, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.19
This classifies the protein as stable.
Aliphatic index: 90.30
Grand average of hydropathicity (GRAVY): -0.023