ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRSRSNSGVR LDGYARLVQQ TILCYQNPVT GLLSASHDQK DAWVRDNIYS ILAVWGLGMA 

        70         80         90        100        110        120 
YRKNADRDED KAKAYELEQN VVKLMRGLLQ CMMRQVDKVE KFKHTQSTKD SLHAKYNTAT 

       130        140        150        160        170        180 
CSTVVGDDQW GHLQVDATSL FLLFLAQMTA SGLRIIFTLD EVAFIQNLVF YIEAAYKVAD 

       190        200        210        220        230        240 
YGMWERGDKT NQGIPELNAS SVGMAKAALE AIDELDLFGA HGGRKSVIHV LPDEVEHCQS 

       250        260        270        280        290        300 
ILFSMLPRAS TSKEIDAGLL SIISFPAFAV EDVHLVNVTK NEIISKLQGR YGCCRFLRDG 

       310        320        330        340        350        360 
YKTPREDPHR LHYDPAELKL FENIECEWPV FWTYLIIDGV FNGDAVQVQE YREALEGILI 

       370        380        390        400        410        420 
RGKDGIHLVP ELYAIPPDKV DEEYKNPHTV DRIPLGKLPH LWGQSLYILS SLLAEGFLAT 

       430        440        450        460        470        480 
GEIDPLNRRF STSVKPDVVV QVAVLAENSH IKGLLKQHGI NVQSIADVHP IRVQPGRILS 

       490        500        510        520        530        540 
HIYAKLGQNK NMKLSGRPYR HIGVLGTSKL YVIRNHIFTF TPQFTDQHHF YLALDNEMIV 

       550        560        570        580        590        600 
EMLRIELAYL CTCWRMTGRP TLTFPVTHTM LTNDGSDIHP AVLSTIRKLE DGYFGGARVK 

       610        620        630        640        650        660 
LGILAEFLTT SFYTYLTFLD PDCDEKLFGD ISDRSFSPDS ESDLGGYLED SSHQESQDEL 

       670        680        690        700        710        720 
DQYISHLLQS TSLKCYLPPL CKKSEDSHVF SAVHSTRDIL SVMAKAKGLE TPFFPMILPT 

       730        740        750        760        770        780 
RVLSGHRKSL NLVDSPQPFL KKTPEYDYQW PRDDQDEVDC EKLVGQLKDC SNLQDQADIL 

       790        800        810        820        830        840 
YILYVIKGPR WDTNLFGQHG VTVHSLLGEL YGKAGLNQEW SLIRYISGLL RKKVEVLAEA 

       850        860        870        880        890        900 
CADLLSHQKQ LTVGLPPEPR EKTISTPLPP EELTELIYEA SGQDISIAVL TQEIVVYLAM 

       910        920        930        940        950        960 
YVRAQPSLFA EMLRLRIGLI IQVMATELAR SLNCSGEEAS ESLMNLSPFD MKSLLHHILS 

       970        980        990       1000       1010       1020 
GKEFGVERSV RPIHSSMSSP AISIHEVGHT GVTKTERSGI SRLRSEMKQM NRRASADEQF 

      1030       1040       1050       1060       1070       1080 
FPLGQAVPKR LHSIKSVRSS TPSSPTGTSS TDSGGHHLGW GEQQGQWLRR RRLDGAINRV 

      1090       1100       1110       1120       1130       1140 
PVGFYQRVWK ILQKCHGLSI DGYVLPSSTT QEMTPCEIKF AVHVESVLNR VSQPEYRQLL 

      1150       1160       1170       1180       1190       1200 
VEAIMVLTLL SDTEMDSIGG IIHVDQIVQL ANQLFLQDQV SFGTTDILEK DQATGICHLF 

      1210       1220       1230 
YDSAPSGAYG TMTYLTKAVA SHLQDLLPSS GCQMQ 

References and documentation are available.

Number of amino acids: 1235

Molecular weight: 138589.50

Theoretical pI: 5.98

Amino acid composition: 
Ala (A)  71	  5.7%
Arg (R)  63	  5.1%
Asn (N)  32	  2.6%
Asp (D)  74	  6.0%
Cys (C)  20	  1.6%
Gln (Q)  63	  5.1%
Glu (E)  74	  6.0%
Gly (G)  82	  6.6%
His (H)  44	  3.6%
Ile (I)  74	  6.0%
Leu (L) 145	 11.7%
Lys (K)  60	  4.9%
Met (M)  29	  2.3%
Phe (F)  42	  3.4%
Pro (P)  56	  4.5%
Ser (S) 101	  8.2%
Thr (T)  65	  5.3%
Trp (W)  14	  1.1%
Tyr (Y)  43	  3.5%
Val (V)  83	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 148
Total number of positively charged residues (Arg + Lys): 123

Atomic composition:

Carbon      C	      6184
Hydrogen    H	      9745
Nitrogen    N	      1681
Oxygen      O	      1836
Sulfur      S	        49

Formula: C6184H9745N1681O1836S49
Total number of atoms: 19495

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   142320
Abs 0.1% (=1 g/l)   1.027, assuming all pairs of Cys residues form cystines


Ext. coefficient   141070
Abs 0.1% (=1 g/l)   1.018, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.75
This classifies the protein as unstable.



Aliphatic index: 94.40

Grand average of hydropathicity (GRAVY): -0.197