ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS APPVSGGNYS 

        70         80         90        100        110        120 
HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK TGEPPNNYIT HVRIIEDSKF 

       130        140        150        160        170        180 
PSSRPPPDSK LENKKKRLLI LSAKPNNAKL IQIHKARENS DGSFQIGRTW QLTELVRVEK 

       190        200        210        220        230        240 
DLEISEGFIL TMSKKYYWET NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE 

       250        260        270        280        290        300 
RSYQRAVITN RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA 

       310        320        330        340        350        360 
SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR LQLQKENEMK 

       370        380        390        400        410        420 
RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK QMELKRQRQF EEEQRLKKER 

       430        440        450        460        470        480 
ELLEIQRKQR EQETAERLKK EEQEALAKKE EEEKSKRNKV DNESYTQEIN GKVDNLLEDL 

       490        500        510        520        530        540 
NAVLAEETET TPTMQNGTYV PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA 

       550        560        570        580        590        600 
GLNTSNLSGE DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP 

       610        620        630        640        650        660 
EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID LRLAETEYLF 

       670        680        690        700        710        720 
NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS NFSNDIENVE SQDNGLQVES 

       730        740        750        760        770        780 
ANKKLLWNTL DELLKTVSLD EISLNQLLEC PIREKNLPWM ENQLNLLLKA FQAIGSDGNE 

       790        800        810        820        830        840 
VEYNLREISG LKQRLQFYEK VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF 

       850        860        870        880        890        900 
SPLILFCKEI SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL 

       910        920        930        940        950        960 
NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI SSKHNFEEYI 

       970        980        990       1000       1010       1020 
KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI VTRLSSYFVE LVKAEPTVAP 

      1030       1040       1050       1060       1070       1080 
ALTFYLENEI KSLESSNHEF LLSAVTRMYT QIKQVWSDNV EEQVLHFERI SNATTNGEIL 

      1090       1100       1110       1120       1130       1140 
PGILDLPVGL KNSEDLFQFA KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN 

      1150       1160       1170       1180       1190       1200 
SSINPNLSDT AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK 

      1210       1220       1230       1240       1250       1260 
ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN ILLAYTSHEI 

      1270       1280       1290       1300       1310       1320 
ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV SLYLKLYTVI DKHYRGTNIR 

      1330 
FTKNDIISAF EEYKNA 

References and documentation are available.

Number of amino acids: 1336

Molecular weight: 154694.92

Theoretical pI: 5.34

Amino acid composition: 
Ala (A)  57	  4.3%
Arg (R)  73	  5.5%
Asn (N) 102	  7.6%
Asp (D)  60	  4.5%
Cys (C)   9	  0.7%
Gln (Q)  69	  5.2%
Glu (E) 150	 11.2%
Gly (G)  36	  2.7%
His (H)  28	  2.1%
Ile (I)  86	  6.4%
Leu (L) 132	  9.9%
Lys (K)  94	  7.0%
Met (M)  28	  2.1%
Phe (F)  49	  3.7%
Pro (P)  46	  3.4%
Ser (S) 124	  9.3%
Thr (T)  75	  5.6%
Trp (W)  13	  1.0%
Tyr (Y)  37	  2.8%
Val (V)  68	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 210
Total number of positively charged residues (Arg + Lys): 167

Atomic composition:

Carbon      C	      6790
Hydrogen    H	     10787
Nitrogen    N	      1889
Oxygen      O	      2164
Sulfur      S	        37

Formula: C6790H10787N1889O2164S37
Total number of atoms: 21667

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   127130
Abs 0.1% (=1 g/l)   0.822, assuming all pairs of Cys residues form cystines


Ext. coefficient   126630
Abs 0.1% (=1 g/l)   0.819, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.14
This classifies the protein as unstable.



Aliphatic index: 82.66

Grand average of hydropathicity (GRAVY): -0.695