ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRSVVLVALA AAIGNMLQGW DNATIAGAVI YIKKEFHLEK EPKIEGLIVA MSLIGATLIT 

        70         80         90        100        110        120 
TFSGPVSDKV GRRSMLILSS VLYFLSSIVM FWSPNVYVLL FARLLDGFGI GLAVTLVPIY 

       130        140        150        160        170        180 
ISETAPSEIR GLLNTFPQFC GSGGMFLSYC LVFGMSLQES PSWRLMLGVL SIPSIAYFVL 

       190        200        210        220        230        240 
AAFFLPESPR WLVSKGRMDE ARQVLQRLRG REDVSGELAL LVEGLGVGKD TSIEEYVIGP 

       250        260        270        280        290        300 
DNEENEGGNE LPRKDQIKLY GPEDGQSWMA KPVKGQSSLA LASRQGSMLP RGGSLMDPLV 

       310        320        330        340        350        360 
TLFGSIHENL PSENMNASSR SMLFPNMGSI LGMMGRQESQ WDPERNNEDS SDQDENLNSP 

       370        380        390        400        410        420 
LLSPQTTEPD DYHQRTVGTM HRRQSSLFMA NVGETATATS IGGGWQLAWK YNDKVGADGK 

       430        440        450        460        470        480 
RVNGGLQRMY IHEETANNNT NNIPFSRRGS LLSFHPEGDG HDQVNGYVQA AALVSQASMM 

       490        500        510        520        530        540 
PGGKGETAML PKEVKDGPGW RELKEPGVKR ALMVGVGLQI LQQFAGINGV MYYTPQILEE 

       550        560        570        580        590        600 
TGVSSLLTNL GISAESASLL ISALTTLLML PCILVSMRSL MLSTIPILIL SLVTLVIGSL 

       610        620        630        640        650        660 
VNLGGSINAL ISTASVTVYL SCFVMGFGAI PNILCSEIFP TSVRGLCITI CALTFWICDI 

       670        680        690        700        710        720 
IVTYTLPVML KSIGIAGVFG IYAIVCAVAW VFVYLKVPET KGMPLEVISE FFSVGAKQQD 


AAASFLSDG 

References and documentation are available.

Number of amino acids: 729

Molecular weight: 78828.37

Theoretical pI: 5.25

Amino acid composition: 
Ala (A)  49	  6.7%
Arg (R)  29	  4.0%
Asn (N)  29	  4.0%
Asp (D)  24	  3.3%
Cys (C)   9	  1.2%
Gln (Q)  26	  3.6%
Glu (E)  41	  5.6%
Gly (G)  72	  9.9%
His (H)   7	  1.0%
Ile (I)  49	  6.7%
Leu (L)  88	 12.1%
Lys (K)  23	  3.2%
Met (M)  31	  4.3%
Phe (F)  28	  3.8%
Pro (P)  36	  4.9%
Ser (S)  70	  9.6%
Thr (T)  34	  4.7%
Trp (W)  11	  1.5%
Tyr (Y)  18	  2.5%
Val (V)  55	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      3532
Hydrogen    H	      5615
Nitrogen    N	       919
Oxygen      O	      1037
Sulfur      S	        40

Formula: C3532H5615N919O1037S40
Total number of atoms: 11143

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    87820
Abs 0.1% (=1 g/l)   1.114, assuming all pairs of Cys residues form cystines


Ext. coefficient    87320
Abs 0.1% (=1 g/l)   1.108, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.47
This classifies the protein as unstable.



Aliphatic index: 101.89

Grand average of hydropathicity (GRAVY): 0.236