ProtParam
User-provided sequence:
10 20 30 40 50 60
MRTIENSNSI VKKMISIGGC DYSTNFTSVV SNQTIRRVFI ESIIALLIEH NLDGVDLFWR
70 80 90 100 110 120
WVPAALQSEF CSFLKELKNE LMNQEKQYIL SVGAPPAGIE NLEDGYDIEE IIRHVDFVNV
130 140 150 160 170 180
YSMDYAGPWD NQWGTPTGPS APLYGGLDAR RNFNVDYTIK SYIRNIRRPE KFNLAIPFSV
190 200 210 220 230 240
RLWRNVEDAI QPGIEVFRNV TLQDDRAIGE ASKANYVKSK NLGGVWIWTM EHDDDNDTLI
250 260
EAVSSQFGTP CDEQIPFEVI ASANNL
References and
documentation are available.
Number of amino acids: 266
Molecular weight: 30207.03
Theoretical pI: 4.70
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1352
Hydrogen H 2079
Nitrogen N 361
Oxygen O 410
Sulfur S 8
Formula: C
1352H
2079N
361O
410S
8
Total number of atoms: 4210
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52035
Abs 0.1% (=1 g/l) 1.723, assuming all pairs of Cys residues form cystines
Ext. coefficient 51910
Abs 0.1% (=1 g/l) 1.718, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.56
This classifies the protein as unstable.
Aliphatic index: 89.77
Grand average of hydropathicity (GRAVY): -0.273