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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRTIENSNSI VKKMISIGGC DYSTNFTSVV SNQTIRRVFI ESIIALLIEH NLDGVDLFWR

70 80 90 100 110 120
WVPAALQSEF CSFLKELKNE LMNQEKQYIL SVGAPPAGIE NLEDGYDIEE IIRHVDFVNV

130 140 150 160 170 180
YSMDYAGPWD NQWGTPTGPS APLYGGLDAR RNFNVDYTIK SYIRNIRRPE KFNLAIPFSV

190 200 210 220 230 240
RLWRNVEDAI QPGIEVFRNV TLQDDRAIGE ASKANYVKSK NLGGVWIWTM EHDDDNDTLI

250 260
EAVSSQFGTP CDEQIPFEVI ASANNL


References and documentation are available.

Number of amino acids: 266 Molecular weight: 30207.03 Theoretical pI: 4.70
Amino acid composition: 
Ala (A) 16 6.0% Arg (R) 14 5.3% Asn (N) 21 7.9% Asp (D) 18 6.8% Cys (C) 3 1.1% Gln (Q) 9 3.4% Glu (E) 19 7.1% Gly (G) 16 6.0% His (H) 3 1.1% Ile (I) 25 9.4% Leu (L) 18 6.8% Lys (K) 10 3.8% Met (M) 5 1.9% Phe (F) 12 4.5% Pro (P) 12 4.5% Ser (S) 19 7.1% Thr (T) 11 4.1% Trp (W) 7 2.6% Tyr (Y) 9 3.4% Val (V) 19 7.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 24 Atomic composition: Carbon C 1352 Hydrogen H 2079 Nitrogen N 361 Oxygen O 410 Sulfur S 8 Formula: C1352H2079N361O410S8 Total number of atoms: 4210 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 52035 Abs 0.1% (=1 g/l) 1.723, assuming all pairs of Cys residues form cystines Ext. coefficient 51910 Abs 0.1% (=1 g/l) 1.718, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 50.56 This classifies the protein as unstable. Aliphatic index: 89.77 Grand average of hydropathicity (GRAVY): -0.273