ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRTSDSHLPL SNLARSDSIE FKDAVINEKW VHSANRRKGH LTPKAPEKKS GWFGGQKSEE 

        70         80         90        100        110        120 
ELLMERVEQH ELEELQTFIA QKLFRVDGII CDEETRILLV EKLMNAKEYP TINHDELAHR 

       130        140        150        160        170        180 
YGSSMAGWLR DRLVPSMSDC SSVLQRAAAE FYQNKMSDPL CNWGQLNPEH VSMVAARIAK 

       190        200        210        220        230        240 
FSEEMSSKVK WSLLVEPGKF SCHLTEFVQE FNRLDRMFVS NELSDEESLQ TAFNANYLTK 

       250        260        270        280        290        300 
ARSKMVPCAE FSRVKLNDGL GRLDDRNELR NGMFSDEHEF LQEEGYTAKS TYGTTDFIHA 

       310        320        330        340        350        360 
NYVKGGPLLN TFICAQAPLK NTQEDFWRMV FQEKCQFIVM LNSAVDSSTL GPLDSANRNH 

       370        380        390        400        410        420 
CPYYWPRAEN ESLRFGSFHI TCMKVDSKAD PLFTITKLKV QKVGGNLLDA EFDEELFLEH 

       430        440        450        460        470        480 
WQWDWQYLGD VHWPFRVLRK ARQLSTPTIV QCIDGCSKSG TLVSIETALM HFIRGSPITK 

       490        500        510        520        530        540 
SLILQSCVFV RLQRRLSVSS VLLYLFIYRV ILRWIEPYVN KWYHRAALGL RFKSIGFIQK 

       550 
YNAMIQEFSR ITPAY 

References and documentation are available.

Number of amino acids: 555

Molecular weight: 64151.38

Theoretical pI: 6.97

Amino acid composition: 
Ala (A)  33	  5.9%
Arg (R)  35	  6.3%
Asn (N)  25	  4.5%
Asp (D)  26	  4.7%
Cys (C)  12	  2.2%
Gln (Q)  23	  4.1%
Glu (E)  43	  7.7%
Gly (G)  26	  4.7%
His (H)  16	  2.9%
Ile (I)  26	  4.7%
Leu (L)  58	 10.5%
Lys (K)  33	  5.9%
Met (M)  16	  2.9%
Phe (F)  31	  5.6%
Pro (P)  20	  3.6%
Ser (S)  47	  8.5%
Thr (T)  24	  4.3%
Trp (W)  13	  2.3%
Tyr (Y)  16	  2.9%
Val (V)  32	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      2872
Hydrogen    H	      4450
Nitrogen    N	       786
Oxygen      O	       829
Sulfur      S	        28

Formula: C2872H4450N786O829S28
Total number of atoms: 8965

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    96090
Abs 0.1% (=1 g/l)   1.498, assuming all pairs of Cys residues form cystines


Ext. coefficient    95340
Abs 0.1% (=1 g/l)   1.486, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.35
This classifies the protein as unstable.



Aliphatic index: 81.69

Grand average of hydropathicity (GRAVY): -0.356