ProtParam
User-provided sequence:
10 20 30 40 50 60
MRTVQAISGL VTSRFFGTST RIMASGKTLN TSNINPNVIK MEYAVRGPIV IRAVELEKEL
70 80 90 100 110 120
ATGAQKPFPN VIKANIGDAH AMGQKPITFI RQLLACIVNP EIMKTDKSIP SDVIEHANAF
130 140 150 160 170 180
LGSCGGKSAG AYSQSTGVEI VRKHVAEYIK RRDGGIPCNS EDVCLSGGAS ESIRNVLKLF
190 200 210 220 230 240
INHNNAKKVG VMIPIPQYPL YSATIEEFGL GQVGYYLSES SNWSMDEAEL ERSFNDHCKE
250 260 270 280 290 300
YDIRVLCIIN PGNPTGQALS RENIETIIKF AQKKNLFLMA DEVYQDNVYA QGSQFHSFKK
310 320 330 340 350
VLVEMGEPYN KMELASFHSV SKGYMGECGM RGGYVEFLNL DPEVTRTSRH
References and
documentation are available.
Number of amino acids: 350
Molecular weight: 38677.26
Theoretical pI: 7.60
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1707
Hydrogen H 2707
Nitrogen N 471
Oxygen O 515
Sulfur S 19
Formula: C
1707H
2707N
471O
515S
19
Total number of atoms: 5419
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25245
Abs 0.1% (=1 g/l) 0.653, assuming all pairs of Cys residues form cystines
Ext. coefficient 24870
Abs 0.1% (=1 g/l) 0.643, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.72
This classifies the protein as stable.
Aliphatic index: 83.29
Grand average of hydropathicity (GRAVY): -0.231