ProtParam
User-provided sequence:
10 20 30 40 50 60
MRVSTSVNGG VGIVSACTRY CVADPELLLE SLAEVKHSAM TRRIRDVEDE RHRQVKPNKQ
70 80 90 100 110 120
AKDRLETIVN LPSSQVLTRE QRDLVWKFRH YLRQFPKALN KYLRSVNWVH PQEVKTALAL
130 140 150 160 170 180
MNDWELIEAE DALELLSSAF THPAVRAYSV SRLLEAASPE QVLLYLPQLV QALKYEQGQQ
190 200 210 220 230 240
LPEEGNPVPV VSEEEGKIPS VATTPTEELE GRDMTVVTKK EARKAASGDL ATFLIDYALA
250 260 270 280 290 300
SPKVSNYLYW HLKTEIESTK ESKEEHSKMY QNIQDRLMEA LVKRPDTRAQ VDSLHQQQIF
310 320 330 340 350 360
VEDLIILMNE AKARGGRLNE SKSAEFRTML SRAKHMLDLK GVHLPLDPSF RLSSVIPDTA
370 380 390 400 410 420
SFFKSEMMPA KISFKVLQPN GKADRNIPEE YTVIFKTGDD LRQDQLIQQM VRLIDIILKK
430 440 450 460 470 480
GQLDLKLTPY LVLSTGVGQG FVQCIKSKPL RAIQEQYKAH KMDCIREAMK ELRPGDGPFG
490 500 510 520 530 540
IEPNVIDNYV RSLAGYSVIM YILGLGDRHL DNLLLCENGK LFHVDFGFIL GRDPKPMPPP
550 560 570 580 590 600
MKLTSEMVQV MGGVKSKQFL EFVQHVDSAY RILRRHSNVL LNLFSLMLDA GIPDIAAEPD
610 620 630 640 650
KAIFKIEQRL RLDLSDEAAT KHIFTQIESS LNAKMAMISD IIHAYKQNLM
References and
documentation are available.
Number of amino acids: 650
Molecular weight: 73799.35
Theoretical pI: 7.29
Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 85
Atomic composition:
Carbon C 3286
Hydrogen H 5294
Nitrogen N 906
Oxygen O 963
Sulfur S 28
Formula: C
3286H
5294N
906O
963S
28
Total number of atoms: 10477
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49070
Abs 0.1% (=1 g/l) 0.665, assuming all pairs of Cys residues form cystines
Ext. coefficient 48820
Abs 0.1% (=1 g/l) 0.662, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.39
This classifies the protein as unstable.
Aliphatic index: 96.00
Grand average of hydropathicity (GRAVY): -0.297