ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRYNNYDNSG SSFLTRVVKK SDMEKTLLLN REIDDWKSND KKKAYKERGR VYASCSFIEV 

        70         80         90        100        110        120 
SFSQIRAVDV EKKIENAEQL RDLTRNIVKN KTSSLNEITP SKNRVTSACN SERRTTSQEA 

       130        140        150        160        170        180 
NNLEGYHSCA QGTSRSASIT KKYSKKTTSR PKREKRQTIL PNGEIKECSK CKDTWTIQWR 

       190        200        210        220 
SGPDQNRELC SPCGLAYGKR LKKENEKKRQ AADKRIDRNN P 

References and documentation are available.

Number of amino acids: 221

Molecular weight: 25513.74

Theoretical pI: 9.81

Amino acid composition: 
Ala (A)  11	  5.0%
Arg (R)  21	  9.5%
Asn (N)  18	  8.1%
Asp (D)  11	  5.0%
Cys (C)   7	  3.2%
Gln (Q)   8	  3.6%
Glu (E)  17	  7.7%
Gly (G)   8	  3.6%
His (H)   1	  0.5%
Ile (I)  11	  5.0%
Leu (L)  12	  5.4%
Lys (K)  28	 12.7%
Met (M)   2	  0.9%
Phe (F)   3	  1.4%
Pro (P)   6	  2.7%
Ser (S)  24	 10.9%
Thr (T)  15	  6.8%
Trp (W)   3	  1.4%
Tyr (Y)   7	  3.2%
Val (V)   8	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      1084
Hydrogen    H	      1787
Nitrogen    N	       343
Oxygen      O	       350
Sulfur      S	         9

Formula: C1084H1787N343O350S9
Total number of atoms: 3573

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27305
Abs 0.1% (=1 g/l)   1.070, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   1.056, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.05
This classifies the protein as unstable.



Aliphatic index: 56.06

Grand average of hydropathicity (GRAVY): -1.231