ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRYQLFRWLY GLIATVDNEP LPLQIKSEEE AFGENKKKKQ LASLENAISP GKLDSVAIRT 

        70         80         90        100        110        120 
VPSANANAMG NAGSAEIVSN QIIRRREMST MGKETESHSI PISEGQDAEH ILYRLKRGSK 

       130        140        150        160        170        180 
LSVHPDASLL GRKIVLYTNY PAEGQKFVRT EYRVLGWQLS NGKQITSVMH PEAHVVDTDI 

       190        200        210        220        230        240 
RSQVELNMSG TYHFYFRYLE RPDTGCSGAD GALYVQVEPT LHVGPPGAQK TIPLDSVRCQ 

       250        260        270        280        290        300 
TVLAKLLGPL DTWEPKLRVA KEAGYNVIHF TPIQELGGSR SCYSLRDQLK VNSHFAPQKG 

       310        320        330        340        350        360 
GKISFEDVEK VIKKCRQEWG VASICDIVLN HTANESDWLL QHPDATYSCA TCPYLRPAFL 

       370        380        390        400        410        420 
LDATFAQCGA DIAEGSLEHV GVPAVIEQEC HLEALKYQLH TSYMSKVNIH ELYQCDVMKY 

       430        440        450        460        470        480 
VNEFMSQVRT REPPKNVANE CRFQEIQLIQ DPQYRRLAST INFELALEIF NAFHGDCFDE 

       490        500        510        520        530        540 
ESRFRKCAET LRRHLDALND RVRCEVQGYI NYAIDNVLAG VRYERVQGDG PRVKEISEKH 

       550        560        570        580        590        600 
SVFMVYFTHT GTQGKSLTEI EADMYTKAGE FFMAHNGWVM GYSDPLRDFA EEQPGRANVY 

       610        620        630        640        650        660 
LKRELISWGD SVKLRFGRRP EDSPYLWQHM TEYVQTTARI FDGVRLDNCH STPLHVAEYL 

       670        680        690        700        710        720 
LDAARKINPE LYVVAELFTN SDYTDNVFVN RLGITSLIRE ALSAWDSHEQ GRLVYRYGGV 

       730        740        750        760        770        780 
PVGGFQANSS RHEATSVAHA LFLDLTHDNP SPVEKRSVYD LLPSAALVSM ACCATGSNRG 

       790        800        810        820        830        840 
YDELVPHHIH VVDEERTYQE WGKGVDSKSG IMGAKRALNL LHGQLAEEGF SQVYVDQMDP 

       850        860        870        880        890        900 
NVVAVTRHSP ITHQSVILVA HTAFGYPSPN AGPTGIRPLR FEGVLDEIIL EASLTMQSDK 

       910        920        930        940        950        960 
PFDRPAPFKK DPNVINGFTQ FQLNLQEHIP LAKSTVFQTQ AYSDGNNTEL NFANLRPGTV 

       970        980        990       1000       1010       1020 
VAIRVSMHPG PRTSFDKLQK ISAALRIGSG EEYSQLQAIV SKLDLVALSG ALFSCDDEER 

      1030       1040       1050       1060       1070       1080 
DLGKGGTAYD IPNFGKIVYC GLQGFISLLT EISPKNDLGH PLCNNLRDGN WMMDYISDRL 

      1090       1100       1110       1120       1130       1140 
TSYEDLKPLS AWFKATFEPL KNIPRYLIPC YFDAIVSGVY NVLINQVNEL MPDFIKNGHS 

      1150       1160       1170       1180       1190       1200 
FPQSLALSTL QFLSVCKSAN LPGFSPALSP PKPPKQCVTL SAGLPHFSTG YMRCWGRDTF 

      1210       1220       1230       1240       1250       1260 
IALRGSMFLT GRYNEARFII IGFGQTLRHG LIPNLLDSGS KPRFNCRDAI WWWMYCIKQY 

      1270       1280       1290       1300       1310       1320 
VEDAPKGAEI LKDKVSRIFP YDDADAHAPG AFDQLLFDVM QEALQVHFQG LQYRERNAGY 

      1330       1340       1350       1360       1370       1380 
EIDAHMVDQG FNNQIGIHPE TGFVFGGNNF NCGTWMDKMG SSQKAGNKGR PSTPRDGSAV 

      1390       1400       1410       1420       1430       1440 
ELVGLQYAVL RFMQSLAEKE VIPYTGVERK GPSGEVTKWS YKEWADRIKN NFDKYFFVSE 

      1450       1460       1470       1480       1490       1500 
SETCSVANKK LIYKDSYGAT QSWTDYQLRC NFPITLTVAP DLCNPQNAWR ALERAKKYLL 

      1510       1520       1530       1540       1550       1560 
GPLGMKTMDP EDWNYRANYD NSNDSTDCTV AHGANYHQGP EWVWPIGFYL RARLIFAKKC 

      1570       1580       1590       1600       1610       1620 
GHLDETIAET WAILRAHLRE LQTSHWRGLP ELTNDNGSYC GDSCRTQAWS VAAILEVLYD 


LHSLGADVA 

References and documentation are available.

Number of amino acids: 1629

Molecular weight: 183218.54

Theoretical pI: 6.08

Amino acid composition: 
Ala (A) 122	  7.5%
Arg (R)  90	  5.5%
Asn (N)  77	  4.7%
Asp (D)  91	  5.6%
Cys (C)  34	  2.1%
Gln (Q)  71	  4.4%
Glu (E) 101	  6.2%
Gly (G) 116	  7.1%
His (H)  50	  3.1%
Ile (I)  80	  4.9%
Leu (L) 149	  9.1%
Lys (K)  77	  4.7%
Met (M)  32	  2.0%
Phe (F)  69	  4.2%
Pro (P)  84	  5.2%
Ser (S) 110	  6.8%
Thr (T)  78	  4.8%
Trp (W)  27	  1.7%
Tyr (Y)  68	  4.2%
Val (V) 103	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 192
Total number of positively charged residues (Arg + Lys): 167

Atomic composition:

Carbon      C	      8175
Hydrogen    H	     12597
Nitrogen    N	      2251
Oxygen      O	      2418
Sulfur      S	        66

Formula: C8175H12597N2251O2418S66
Total number of atoms: 25507

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   251945
Abs 0.1% (=1 g/l)   1.375, assuming all pairs of Cys residues form cystines


Ext. coefficient   249820
Abs 0.1% (=1 g/l)   1.364, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.67
This classifies the protein as stable.



Aliphatic index: 80.65

Grand average of hydropathicity (GRAVY): -0.352