ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAAIEKETG MLDVGKHCAY CRQLDFLPFH CSFCNEDFCS NHRLKEDHHC RWLLEHEEVH 

        70         80         90        100        110        120 
KTEKSPSKSR DGSSSNDEAY FKSLLPERAS VRIQRVSETR EPLRGSNTAK VSSTLNSKTL 

       130        140        150        160        170        180 
DKIFKFFQRN EKRKSNNKSK KNFGSSSNKI IQLANLKKIA KGDPKIPMQN RIYIWCYLVD 

       190        200        210        220        230        240 
GDETDIAKED TRMPLYINKM WPVGRAMDYL SIQLNVKSST LTNSSSNDKF QLCKLKEGKQ 

       250        260        270 
VSFYNIGASL RVTNEIKDLD TLYLVHNNAD EKSN 

References and documentation are available.

Number of amino acids: 274

Molecular weight: 31516.77

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  12	  4.4%
Arg (R)  15	  5.5%
Asn (N)  21	  7.7%
Asp (D)  17	  6.2%
Cys (C)   8	  2.9%
Gln (Q)   8	  2.9%
Glu (E)  18	  6.6%
Gly (G)  10	  3.6%
His (H)   8	  2.9%
Ile (I)  14	  5.1%
Leu (L)  24	  8.8%
Lys (K)  30	 10.9%
Met (M)   6	  2.2%
Phe (F)  11	  4.0%
Pro (P)   8	  2.9%
Ser (S)  30	 10.9%
Thr (T)  12	  4.4%
Trp (W)   3	  1.1%
Tyr (Y)   8	  2.9%
Val (V)  11	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1375
Hydrogen    H	      2191
Nitrogen    N	       397
Oxygen      O	       424
Sulfur      S	        14

Formula: C1375H2191N397O424S14
Total number of atoms: 4401

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28920
Abs 0.1% (=1 g/l)   0.918, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.902, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.05
This classifies the protein as stable.



Aliphatic index: 70.11

Grand average of hydropathicity (GRAVY): -0.774