ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAAPLDYKK ALEHLKTYSS KDGLSVQELM DSTTRGGLTY NDFLVLPGLV NFPSSAVSLQ
70 80 90 100 110 120
TKLTKKITLN TPFVSSPMDT VTEADMAIYM ALLGGIGFIH HNCTPKEQAS MVKKVKMFEN
130 140 150 160 170 180
GFINSPIVIS PTTTVGEVKV MKRKFGFSGF PVTEDGKCPG KLVGLVTSRD IQFLEDDSLV
190 200 210 220 230 240
VSEVMTKNPV TGIKGITLKE GNEILKQTKK GKLLIVDDNG NLVSMLSRAD LMKNQNYPLA
250 260 270 280 290 300
SKSATTKQLL CGAAIGTIEA DKERLRLLVE AGLDVVILDS SQGNSVFQLN MIKWIKETFP
310 320 330 340 350 360
DLEIIAGNVA TREQAANLIA AGADGLRIGM GSGSICITQE VMACGRPQGT AVYNVCQFAN
370 380 390 400 410 420
QFGVPCMADG GVQNIGHITK ALALGSSTVM MGGMLAGTTE SPGEYFYKDG KRLKAYRGMG
430 440 450 460 470 480
SIDAMQKTGN KGNASTSRYF SESDSVLVAQ GVSGAVVDKG SIKKFIPYLY NGLQHSCQDI
490 500 510 520
GCESLTSLKE NVQNGEVRFE FRTASAQLEG GVHNLHSYEK RLYN
References and
documentation are available.
Number of amino acids: 524
Molecular weight: 56393.87
Theoretical pI: 8.39
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 2480
Hydrogen H 4007
Nitrogen N 671
Oxygen O 765
Sulfur S 29
Formula: C
2480H
4007N
671O
765S
29
Total number of atoms: 7952
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26860
Abs 0.1% (=1 g/l) 0.476, assuming all pairs of Cys residues form cystines
Ext. coefficient 26360
Abs 0.1% (=1 g/l) 0.467, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.62
This classifies the protein as stable.
Aliphatic index: 87.06
Grand average of hydropathicity (GRAVY): -0.098