ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH TDAYTLSGSD
70 80 90 100 110 120
PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE CGKCKFCTSG KTNLCGAVRA
130 140 150 160 170 180
TQGKGVMPDG TTRFHNAKGE DIYHFMGCST FSEYTVVADV SVVAIDPKAP LDAACLLGCG
190 200 210 220 230 240
VTTGFGAALK TANVQKGDTV AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF
250 260 270 280 290 300
GATDFVNPKE DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII
310 320 330 340 350 360
GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV EEFITHRRPF
370 380
KEINQAFEDL HNGDCLRTVL KSDEIK
References and
documentation are available.
Number of amino acids: 386
Molecular weight: 41042.05
Theoretical pI: 6.33
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1808
Hydrogen H 2889
Nitrogen N 491
Oxygen O 554
Sulfur S 21
Formula: C
1808H
2889N
491O
554S
21
Total number of atoms: 5763
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23795
Abs 0.1% (=1 g/l) 0.580, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.558, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.35
This classifies the protein as stable.
Aliphatic index: 83.39
Grand average of hydropathicity (GRAVY): -0.049