ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSADFGLIGL AVMGQNLILN AADHGFTVCA YNRTQSKVDH FLANEAKGKS IIGATSIEDF 

        70         80         90        100        110        120 
ISKLKRPRKV MLLVKAGAPV DALINQIVPL LEKGDIIIDG GNSHFPDSNR RYEELKKKGI 

       130        140        150        160        170        180 
LFVGSGVSGG EEGARYGPSL MPGGSEEAWP HIKNIFQSIS AKSDGEPCCE WVGPAGAGHY 

       190        200        210        220        230        240 
VKMVHNGIEY GDMQLICEAY DIMKRLGGFT DKEISDVFAK WNNGVLDSFL VEITRDILKF 

       250        260        270        280        290        300 
DDVDGKPLVE KIMDTAGQKG TGKWTAINAL DLGMPVTLIG EAVFARCLSA LKNERIRASK 

       310        320        330        340        350        360 
VLPGPEVPKD AVKDREQFVD DLEQALYASK IISYAQGFML IREAAATYGW KLNNPAIALM 

       370        380        390        400        410        420 
WRGGCIIRSV FLGQITKAYR EEPDLENLLF NKFFADAVTK AQSGWRKSIA LATTYGIPTP 

       430        440        450        460        470        480 
AFSTALSFYD GYRSERLPAN LLQAQRDYFG AHTFRVLPEC ASDNLPVDKD IHINWTGHGG 


NVSSSTYQA 

References and documentation are available.

Number of amino acids: 489

Molecular weight: 53543.25

Theoretical pI: 6.19

Amino acid composition: 
Ala (A)  47	  9.6%
Arg (R)  21	  4.3%
Asn (N)  21	  4.3%
Asp (D)  31	  6.3%
Cys (C)   7	  1.4%
Gln (Q)  14	  2.9%
Glu (E)  27	  5.5%
Gly (G)  47	  9.6%
His (H)   9	  1.8%
Ile (I)  36	  7.4%
Leu (L)  42	  8.6%
Lys (K)  33	  6.7%
Met (M)  11	  2.2%
Phe (F)  22	  4.5%
Pro (P)  21	  4.3%
Ser (S)  30	  6.1%
Thr (T)  19	  3.9%
Trp (W)   8	  1.6%
Tyr (Y)  15	  3.1%
Val (V)  28	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2402
Hydrogen    H	      3758
Nitrogen    N	       646
Oxygen      O	       705
Sulfur      S	        18

Formula: C2402H3758N646O705S18
Total number of atoms: 7529

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66725
Abs 0.1% (=1 g/l)   1.246, assuming all pairs of Cys residues form cystines


Ext. coefficient    66350
Abs 0.1% (=1 g/l)   1.239, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.63
This classifies the protein as stable.



Aliphatic index: 88.43

Grand average of hydropathicity (GRAVY): -0.143