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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAGDISAIN IKSVKKNRRR KKRRTADVSS SDSSSSDPSS ESEKEEIQNG AIEEHVGENG

70 80 90 100 110 120
KSDHVFSKGN DEDKQEDIAI EVSDVELTDE ESKDLKLNSK EVIDDLTKIS LSKIPEPTKS

130 140 150 160 170 180
QNKEGFMNAS KIAENIKLAR EEYNELAENF VPKGKDKTKL REEYLNLLFE NYGDDINRLR

190 200 210 220
AAPDFTNKSL SILADALQEG IGMFDIGELE LVLKNKEMEN


References and documentation are available.

Number of amino acids: 220 Molecular weight: 24656.31 Theoretical pI: 4.79
Amino acid composition: 
Ala (A) 13 5.9% Arg (R) 9 4.1% Asn (N) 17 7.7% Asp (D) 19 8.6% Cys (C) 0 0.0% Gln (Q) 4 1.8% Glu (E) 29 13.2% Gly (G) 11 5.0% His (H) 2 0.9% Ile (I) 16 7.3% Leu (L) 18 8.2% Lys (K) 25 11.4% Met (M) 4 1.8% Phe (F) 6 2.7% Pro (P) 5 2.3% Ser (S) 24 10.9% Thr (T) 6 2.7% Trp (W) 0 0.0% Tyr (Y) 3 1.4% Val (V) 9 4.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 48 Total number of positively charged residues (Arg + Lys): 34 Atomic composition: Carbon C 1057 Hydrogen H 1723 Nitrogen N 297 Oxygen O 371 Sulfur S 4 Formula: C1057H1723N297O371S4 Total number of atoms: 3452 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.181 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 51.21 This classifies the protein as unstable. Aliphatic index: 78.05 Grand average of hydropathicity (GRAVY): -0.907