ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAGIGESRT ELLTWLNGLL NLNYKKIEEC GTGAAYCQIM DSIYGDLPMN RVKFNATAEY
70 80 90 100 110 120
EFQTNYKILQ SCFSRHGIEK TVYVDKLIRC KFQDNLEFLQ WLKKHWIRHK DESVYDPDAR
130 140 150 160 170 180
RKYRPIITNN SATKPRTVSN PTTAKRSSST GTGSAMSGGL ATRHSSLGIN GSRKTSVTQG
190 200 210 220 230 240
QLVAIQAELT KSQETIGSLN EEIEQYKGTV STLEIEREFY FNKLRDIEIL VHTTQDLINE
250 260 270 280 290 300
GVYKFNDETI TGHGNGNGGA LLRFVKKVES ILYATAEGFE MNDGEDELND KNLGEHGTVP
310 320 330 340
NQGGYANSNG EVNGNEGSNH DVIMQNDEGE VGVSNNLIID EETF
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 38361.63
Theoretical pI: 5.26
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1669
Hydrogen H 2625
Nitrogen N 471
Oxygen O 547
Sulfur S 10
Formula: C
1669H
2625N
471O
547S
10
Total number of atoms: 5322
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36120
Abs 0.1% (=1 g/l) 0.942, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.935, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.08
This classifies the protein as stable.
Aliphatic index: 75.67
Grand average of hydropathicity (GRAVY): -0.616