ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAIKEVKSE LRQFMKTLLG KISKEETQRQ TEAVFEKIIE SKWFQESKRL SVYVSTSGEI
70 80 90 100 110 120
QTDSIIQKAL EMGKEVFIPQ FTKGSTAMDM VRVPDQTAFD NLPSTLWGIR QPEPKWKWQS
130 140 150 160 170 180
YHETGPLDLI LAPGVAFSPY GLRCGHGKGY YDRFFSTHHK HFPENSPKKI GLALREQIIG
190 200
TIPISETDVE LDEVIYEGET IIFDTI
References and
documentation are available.
Number of amino acids: 206
Molecular weight: 23560.94
Theoretical pI: 5.79
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1067
Hydrogen H 1662
Nitrogen N 274
Oxygen O 315
Sulfur S 6
Formula: C
1067H
1662N
274O
315S
6
Total number of atoms: 3324
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30940
Abs 0.1% (=1 g/l) 1.313, assuming all pairs of Cys residues form cystines
Ext. coefficient 30940
Abs 0.1% (=1 g/l) 1.313, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.03
This classifies the protein as unstable.
Aliphatic index: 80.44
Grand average of hydropathicity (GRAVY): -0.414