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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAIQSPAPK PLQPTYPAAS PASTNAYMKP GLIGSPAVSN HTEPNNGNNE TAEPQGPNQR

70 80 90 100 110 120
IDLGAMIEEL TSLLGKESWT KYAQIISLFI LGKLSRKELS NELELVFSPS AASLEKSNTN

130 140 150 160 170 180
HHHSLVRLHN QLLLGIFANS LRENPLGRNG NESSWGFGNG SNNPNNKLKR INKHNSQIEV

190 200 210 220 230 240
YKKIVMSLPL NDRNRLKMIT KEAGKRGFIF CSVFQARLNN IPKIPIVTNP ESLKRVKSNN

250 260 270 280 290 300
LKTPLEWSQD IMNGFNVPLA SESHSLPDTD SFYLRMVGIA REHGLVGTVD ARCVELISLA

310 320 330 340 350 360
LDQYLKNIIE FTIDTVRYRR KKYSDYYDLN ESGLYKSVSE MAADKRDAKI KQLDDDKNED

370 380 390 400 410 420
ECADEAKSIN NGNNSSKDDI GDISMSSITK AGEAVNEELH ENRTISLTNE DIYDSLSIFP

430 440 450 460 470 480
NLVEPSGSYY ALTNLGLVND DELVDMKSNI DDLPDFLNEK PTFTPLDERN VGTRHELNWL


IKGILTED


References and documentation are available.

Number of amino acids: 488 Molecular weight: 54466.21 Theoretical pI: 5.46
Amino acid composition: 
Ala (A) 27 5.5% Arg (R) 21 4.3% Asn (N) 48 9.8% Asp (D) 30 6.1% Cys (C) 3 0.6% Gln (Q) 11 2.3% Glu (E) 36 7.4% Gly (G) 27 5.5% His (H) 10 2.0% Ile (I) 33 6.8% Leu (L) 53 10.9% Lys (K) 33 6.8% Met (M) 10 2.0% Phe (F) 13 2.7% Pro (P) 26 5.3% Ser (S) 47 9.6% Thr (T) 22 4.5% Trp (W) 4 0.8% Tyr (Y) 14 2.9% Val (V) 20 4.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 66 Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 2387 Hydrogen H 3799 Nitrogen N 667 Oxygen O 763 Sulfur S 13 Formula: C2387H3799N667O763S13 Total number of atoms: 7629 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 42985 Abs 0.1% (=1 g/l) 0.789, assuming all pairs of Cys residues form cystines Ext. coefficient 42860 Abs 0.1% (=1 g/l) 0.787, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.19 This classifies the protein as unstable. Aliphatic index: 86.15 Grand average of hydropathicity (GRAVY): -0.562