ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAIQSPAPK PLQPTYPAAS PASTNAYMKP GLIGSPAVSN HTEPNNGNNE TAEPQGPNQR
70 80 90 100 110 120
IDLGAMIEEL TSLLGKESWT KYAQIISLFI LGKLSRKELS NELELVFSPS AASLEKSNTN
130 140 150 160 170 180
HHHSLVRLHN QLLLGIFANS LRENPLGRNG NESSWGFGNG SNNPNNKLKR INKHNSQIEV
190 200 210 220 230 240
YKKIVMSLPL NDRNRLKMIT KEAGKRGFIF CSVFQARLNN IPKIPIVTNP ESLKRVKSNN
250 260 270 280 290 300
LKTPLEWSQD IMNGFNVPLA SESHSLPDTD SFYLRMVGIA REHGLVGTVD ARCVELISLA
310 320 330 340 350 360
LDQYLKNIIE FTIDTVRYRR KKYSDYYDLN ESGLYKSVSE MAADKRDAKI KQLDDDKNED
370 380 390 400 410 420
ECADEAKSIN NGNNSSKDDI GDISMSSITK AGEAVNEELH ENRTISLTNE DIYDSLSIFP
430 440 450 460 470 480
NLVEPSGSYY ALTNLGLVND DELVDMKSNI DDLPDFLNEK PTFTPLDERN VGTRHELNWL
IKGILTED
References and
documentation are available.
Number of amino acids: 488
Molecular weight: 54466.21
Theoretical pI: 5.46
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2387
Hydrogen H 3799
Nitrogen N 667
Oxygen O 763
Sulfur S 13
Formula: C
2387H
3799N
667O
763S
13
Total number of atoms: 7629
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42985
Abs 0.1% (=1 g/l) 0.789, assuming all pairs of Cys residues form cystines
Ext. coefficient 42860
Abs 0.1% (=1 g/l) 0.787, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.19
This classifies the protein as unstable.
Aliphatic index: 86.15
Grand average of hydropathicity (GRAVY): -0.562