ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA TTGDLPPNSK 

        70         80         90        100        110        120 
GGVFIHDPKI TGEKDIRAQV KLAFTSANGL NMIVTRNIQL LMKKTTTTFK TLEGQLVAIN 

       130        140        150        160        170        180 
NSGDRSTLST RSLELDAQVP LYLGVPKAIL EYVIFCHQED SLWPLSEPSN LKKKFDEIFQ 

       190        200        210        220        230        240 
AMKFTKALDN LKSIKKDMSV DIKLLKQSVE HLKLDKDRSK AMKLNIHQLQ TKIDQYNEEV 

       250        260        270        280        290        300 
SEIESQLNEI TEKSDKLFKS NQDFQKILSK VENLKNTKLS ISDQVKRLSN SIDILDLSKP 

       310        320        330        340        350        360 
DLQNLLANFS KVLMDKNNQL RDLETDISSL KDRQSSLQSL SNSLIRRQGE LEAGKETYEK 

       370        380        390        400        410        420 
NRNHLSSLKE AFQHKFQGLS NIENSDMAQV NHEMSQFKAF ISQDLTDTID QFAKDIQLKE 

       430        440        450        460        470        480 
TNLSDLIKSI TVDSQNLEYN KKDRSKLIHD SEELAEKLKS FKSLSTQDSL NHELENLKTY 

       490        500        510        520        530        540 
KEKLQSWESE NIIPKLNQKI EEKNNEMIIL ENQIEKFQDR IMKTNQQADL YAKLGLIKKS 

       550        560        570        580        590        600 
INTKLDELQK ITEKLQNDSR IRQVFPLTQE FQRADLEMDF QKLFINMQKN IAINNKKMHE 

       610        620        630        640        650        660 
LDRRYTNALY NLNTIEKDLQ DNQKSKEKVI QLLSENLPED CTIDEYNDVL EETELSYKTA 

       670        680        690        700        710        720 
LENLKMHQTT LEFNRKALEI AERDSCCYLC SRKFENESFK SKLLQELKTK TDANFEKTLK 

       730        740        750        760        770        780 
DTVQNEKEYL HSLRLLEKHI ITLNSINEKI DNSQKCLEKA KEETKTSKSK LDELEVDSTK 

       790        800        810        820        830        840 
LKDEKELAES EIRPLIEKFT YLEKELKDLE NSSKTISEEL SIYNTSEDGI QTVDELRDQQ 

       850        860        870        880        890        900 
RKMNDSLREL RKTISDLQME KDEKVRENSR MINLIKEKEL TVSEIESSLT QKQNIDDSIR 

       910        920        930        940        950        960 
SKRENINDID SRVKELEARI ISLKNKKDEA QSVLDKVKNE RDIQVRNKQK TVADINRLID 

       970        980        990       1000       1010       1020 
RFQTIYNEVV DFEAKGFDEL QTTIKELELN KAQMLELKEQ LDLKSNEVNE EKRKLADSNN 

      1030       1040       1050       1060       1070       1080 
EEKNLKQNLE LIELKSQLQH IESEISRLDV QNAEAERDKY QEESLRLRTR FEKLSSENAG 

      1090       1100       1110       1120       1130       1140 
KLGEMKQLQN QIDSLTHQLR TDYKDIEKNY HKEWVELQTR SFVTDDIDVY SKALDSAIMK 

      1150       1160       1170       1180       1190       1200 
YHGLKMQDIN RIIDELWKRT YSGTDIDTIK IRSDEVSSTV KGKSYNYRVV MYKQDVELDM 

      1210       1220       1230       1240       1250       1260 
RGRCSAGQKV LASIIIRLAL SETFGANCGV IALDEPTTNL DEENIESLAK SLHNIINMRR 

      1270       1280       1290       1300       1310 
HQKNFQLIVI THDEKFLGHM NAAAFTDHFF KVKRDDRQKS QIEWVDINRV TY 

References and documentation are available.

Number of amino acids: 1312

Molecular weight: 152569.01

Theoretical pI: 5.78

Amino acid composition: 
Ala (A)  49	  3.7%
Arg (R)  59	  4.5%
Asn (N)  92	  7.0%
Asp (D)  97	  7.4%
Cys (C)   9	  0.7%
Gln (Q)  79	  6.0%
Glu (E) 132	 10.1%
Gly (G)  29	  2.2%
His (H)  22	  1.7%
Ile (I) 101	  7.7%
Leu (L) 153	 11.7%
Lys (K) 149	 11.4%
Met (M)  27	  2.1%
Phe (F)  39	  3.0%
Pro (P)  14	  1.1%
Ser (S) 108	  8.2%
Thr (T)  73	  5.6%
Trp (W)   5	  0.4%
Tyr (Y)  28	  2.1%
Val (V)  47	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 229
Total number of positively charged residues (Arg + Lys): 208

Atomic composition:

Carbon      C	      6661
Hydrogen    H	     10884
Nitrogen    N	      1858
Oxygen      O	      2151
Sulfur      S	        36

Formula: C6661H10884N1858O2151S36
Total number of atoms: 21590

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69720
Abs 0.1% (=1 g/l)   0.457, assuming all pairs of Cys residues form cystines


Ext. coefficient    69220
Abs 0.1% (=1 g/l)   0.454, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.28
This classifies the protein as unstable.



Aliphatic index: 89.63

Grand average of hydropathicity (GRAVY): -0.781