ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAKAISEQT GKEFLYKHIC TSASVQNRFR YANVTAETDW ERLVQDHPWL LTERLVVKPD 

        70         80         90        100        110        120 
QLIKRRGKLG LVGVNLDLNG VKEWLKTRLM QETMVGKAKG ILKNFLIEPF VAHKQEEEFY 

       130        140        150        160        170        180 
VCIYATREGD YMLFHHEGGV DVGDVDSKAL KLLVGVDEKI SADTVKSQLL THAPADKKEI 

       190        200        210        220        230        240 
LSSFLVGLFN LYEDLYFTYL EINPLVVTKD GVYVLDMAAK IDATADYLCK AKWGDVEFPP 

       250        260        270        280        290        300 
PFGREAYPEE AYIADLDAKS GASLKLTLLN PRGRIWTMVA GGGASVVYSD TICDLGGVNE 

       310        320        330        340        350        360 
LANYGEYSGA PSEQQTYDYA KTILSLMTRE KHPDGKVLII GGSIANFTNV AATFKGIVRA 

       370        380        390        400        410        420 
FKDYQDPLKE HEVTIFVRRG GPNYQEGLRV MGEVGKTTGI PIHVFGTETH MTAIVGMALG 

       430        440        450        460        470        480 
HRPIATQPRG AAHTANFLLN ASGGTTTPAS SRSASFSEVR TGAESSSSHK SRAGLPPAKS 

       490        500        510        520        530        540 
TTLFSNLTKS IVWGMQTRAV QGMLDFDYVC SRQEPSVAAM VYPFTGDHKQ KFYWGHKEIL 

       550        560        570        580        590        600 
LPVYKNMADA MKKHPEVDVL ISFASLRSAY DSTIETMQFP QIHTIAIIAE GIPEALTRKI 

       610        620        630        640        650        660 
IKMADEKGIT IIGPATVGGI KPGCFKIGNT GGMLDNILAS KLYRPGSVAY VSRSGGMSNE 

       670        680        690        700        710        720 
LNNIISRTTD GVYEGVAIGG DRYPGSVFID HVLRYQDTPG VKMIVVLGEI GGNEEYKICN 

       730        740        750        760        770        780 
AIKEGRITKP VVCWCIGTCA TLFSSEVQFG HAGACANQAS ETAVAKNKAL EEAGAFVPKS 

       790        800        810        820        830        840 
FDELGDVIKS VYDNLVAKGV IVPAKELPPP TVPMDYSWAR ELGLIRKPAS FMTSICDERG 

       850        860        870        880        890        900 
QELIYAGMPI TEVFKSEIGL GGTLGLLWFQ RRLPRYACQF IEMCLMVTAD HGPAVSGAHN 

       910        920        930        940        950        960 
TIVCARAGKD LISSLTSGLL TIGDRFGGAL DAAAKQFSKA FDSGMLPMEF VNKMKKDGKL 

       970        980        990       1000       1010       1020 
IMGIGHRVKS INNPDMRVQI LKDFVKQHFP ATQLLDYALE VEKITTSKKP NLILNVDGFI 

      1030       1040       1050       1060       1070       1080 
GVSFVDLLRT CGGFTRDEAD EFVEIGALNG IFVLGRSMGF IGHYLDQKRL KQGLYRHPWD 

      1090 
DISYVLPEHM TM 

References and documentation are available.

Number of amino acids: 1092

Molecular weight: 119709.84

Theoretical pI: 6.83

Amino acid composition: 
Ala (A)  89	  8.2%
Arg (R)  46	  4.2%
Asn (N)  34	  3.1%
Asp (D)  58	  5.3%
Cys (C)  16	  1.5%
Gln (Q)  30	  2.7%
Glu (E)  64	  5.9%
Gly (G) 103	  9.4%
His (H)  26	  2.4%
Ile (I)  72	  6.6%
Leu (L)  94	  8.6%
Lys (K)  73	  6.7%
Met (M)  33	  3.0%
Phe (F)  46	  4.2%
Pro (P)  48	  4.4%
Ser (S)  64	  5.9%
Thr (T)  66	  6.0%
Trp (W)  11	  1.0%
Tyr (Y)  38	  3.5%
Val (V)  81	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 122
Total number of positively charged residues (Arg + Lys): 119

Atomic composition:

Carbon      C	      5368
Hydrogen    H	      8464
Nitrogen    N	      1430
Oxygen      O	      1569
Sulfur      S	        49

Formula: C5368H8464N1430O1569S49
Total number of atoms: 16880

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118120
Abs 0.1% (=1 g/l)   0.987, assuming all pairs of Cys residues form cystines


Ext. coefficient   117120
Abs 0.1% (=1 g/l)   0.978, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.23
This classifies the protein as stable.



Aliphatic index: 88.95

Grand average of hydropathicity (GRAVY): -0.080