ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAKAITEAS GKDILNRHLN THGAGAATCR FSTVNSTTDW SKLAVDHPWL LTTPLVCKPD 

        70         80         90        100        110        120 
QLIKRRGKLG LIGVKKNFEQ VKQWIGERLN KDQKIGNAVG KLRNFIIEPF VPHTDAEEMY 

       130        140        150        160        170        180 
VCIYSHRAAD TILFYHQGGV DIGDVDAKAV KLDVPVNSSL SLADVKSKLL KEVKDAGTKE 

       190        200        210        220        230        240 
RIAKFVSALY TTYVDLYFTY LEINPLVVTA DNLYILDLAA KLDSTADFIC RPKWGEIDYP 

       250        260        270        280        290        300 
PPFGRDAYPE EAYIADLDAK SGASLKLTIL NRNGRIWTMV AGGGASVIYS DTICDLGGAS 

       310        320        330        340        350        360 
ELANYGEYSG APSEQQTYEY AKTILNLMTS SPKHPDGKVL ITGGGIANFT NVAATFQGII 

       370        380        390        400        410        420 
TALREFQPKL VEHNVSIFVR RAGPNYQEGL RKMRDFGSTL GIPLHVFGPE THMTAICGMA 

       430        440        450        460        470        480 
LGKRPIPQTA SVEFSTANFL LPGGQQAQAD LKAASDASEA LGSGSADEAD SAGISGAQRN 

       490        500        510        520        530        540 
GSSLNRKFFS NTTKAIVWGM QQRAVQRDEP SVVAMVYPFT GDHKQKYYWG HKEILIPVYK 

       550        560        570        580        590        600 
KMSDAIHKHK EVDVMVNFAS MRSAYESTLE VLEFPQIRTV AIIAEGIPEN MTRKLIIEAD 

       610        620        630        640        650        660 
KKGVAIIGPA TVGGVKPGCF KIGNTGGMLD NILHSKLYRP GSVAYVSRSG GMSNELNNII 

       670        680        690        700        710        720 
SKATDGVIEG IAIGGDRYPG STFMDHILRY QADPETKLIV LLGEVGGTEE YDVCAALKDG 

       730        740        750        760        770        780 
RITKPLVAWC IGTCASMFTS EVQFGHAGSC ANSDRETATA KNKGLRDAGA YVPDSFDTLG 

       790        800        810        820        830        840 
ELIHHVYGEL VKTGRVVPKE EVPPPTVPMD YSWARELGLI RKPASFMTSI CDERGQELIY 

       850        860        870        880        890        900 
AGMPISEVLS KDVGIGGVIS LLWFQRCLPS YVCKFFEMCL MVTADHGPAV SGAHNTIVCA 

       910        920        930        940        950        960 
RAGKDLVSSV VSGLLTIGDR FGGALDGSAR QFSEAYDTNL HPMEFVNKMR KEGKLILGIG 

       970        980        990       1000       1010       1020 
HRVKSINNPD VRVKIIKEFV LENFPACPLL KYALEVEKIT TNKKPNLILN VDGVIATAFV 

      1030       1040       1050       1060       1070       1080 
DMLRNSGSFT SEEAQEYINV GAINSLFVLG RSIGFIGHYM DQKRLKQGLY RHPWDDISYV 


IPEQYN 

References and documentation are available.

Number of amino acids: 1086

Molecular weight: 118590.88

Theoretical pI: 6.81

Amino acid composition: 
Ala (A)  93	  8.6%
Arg (R)  47	  4.3%
Asn (N)  44	  4.1%
Asp (D)  60	  5.5%
Cys (C)  17	  1.6%
Gln (Q)  29	  2.7%
Glu (E)  60	  5.5%
Gly (G)  99	  9.1%
His (H)  25	  2.3%
Ile (I)  77	  7.1%
Leu (L)  89	  8.2%
Lys (K)  70	  6.4%
Met (M)  26	  2.4%
Phe (F)  40	  3.7%
Pro (P)  50	  4.6%
Ser (S)  71	  6.5%
Thr (T)  60	  5.5%
Trp (W)  11	  1.0%
Tyr (Y)  39	  3.6%
Val (V)  79	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 120
Total number of positively charged residues (Arg + Lys): 117

Atomic composition:

Carbon      C	      5297
Hydrogen    H	      8361
Nitrogen    N	      1431
Oxygen      O	      1570
Sulfur      S	        43

Formula: C5297H8361N1431O1570S43
Total number of atoms: 16702

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   119610
Abs 0.1% (=1 g/l)   1.009, assuming all pairs of Cys residues form cystines


Ext. coefficient   118610
Abs 0.1% (=1 g/l)   1.000, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.20
This classifies the protein as stable.



Aliphatic index: 89.27

Grand average of hydropathicity (GRAVY): -0.121