ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAKQQLRIL VPVKRVVDFQ IKPRVNKTLT GIETSGIKFS INPFDDIAVE EAIRIKEKNK
70 80 90 100 110 120
SLVESTHAVS IGSAKAQDIL RNCLAKGIDT CSLIDSVGKE NIEPLAIAKI LKAVVEKKGS
130 140 150 160 170 180
NLVLMGKQAI DDDCNNTGQM LAGLLNWPQA TNAAKVEFLD NGRVQVTREI DDGEEVIEAS
190 200 210 220 230 240
LPMVITTDLR LNTPRYVGLP KLMKAKKKPI EKLDIAKDFP EINIEPQLKI VSMEEPKTKS
250 260
PGVKLNSVDE LIEKLKEVKA I
References and
documentation are available.
Number of amino acids: 261
Molecular weight: 28758.65
Theoretical pI: 8.59
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1274
Hydrogen H 2143
Nitrogen N 345
Oxygen O 386
Sulfur S 9
Formula: C
1274H
2143N
345O
386S
9
Total number of atoms: 4157
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7115
Abs 0.1% (=1 g/l) 0.247, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 0.243, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.92
This classifies the protein as stable.
Aliphatic index: 107.93
Grand average of hydropathicity (GRAVY): -0.195