ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAKSIREYD GKAVLAYWLN RSPSISKEEY KTVSATPAVQ LAQIQFPLPT LVIPAESTTY 

        70         80         90        100        110        120 
REVVEDVFAK VEQEHPWVKE TKLVAKPDQL IKRRGKSGLL KLNATWDEAK EWIRERAGKN 

       130        140        150        160        170        180 
QKVQHAVGYL TTFLVEPFVP HPPNTEYYIN INSVREGDWI LFCNEGGVDV GDVDAKARKL 

       190        200        210        220        230        240 
LVPVRLSEFP SRATIASTLL SDIPVEQHES LVDFIIRLYS VYVDCQFTYL EINPLVVIPT 

       250        260        270        280        290        300 
AKGADVFYLD LAAKLDQTAE FECGAKWAVA RAPESLGIKT SGEESGAINA DHGPPMVFPA 

       310        320        330        340        350        360 
PFGRELSKEE AYVQGLDAKT GASLKLTILN AEGRVWNLVA GGGASVVYAD AVAVNGAADE 

       370        380        390        400        410        420 
LANYGEYSGA PTDGQTYEYA KTVLDLMTRG EPRADGKVLF IGGGIANFTS PAVTFRAIAR 

       430        440        450        460        470        480 
ALGDYKDKLH AHKVSIWVRR AGPNYQEGLR VIREAGKKFD LPLKVYGPEC HISGIVPMGL 

       490 
GKAPVEAEWQ MA 

References and documentation are available.

Number of amino acids: 492

Molecular weight: 53942.58

Theoretical pI: 5.78

Amino acid composition: 
Ala (A)  55	 11.2%
Arg (R)  23	  4.7%
Asn (N)  15	  3.0%
Asp (D)  24	  4.9%
Cys (C)   4	  0.8%
Gln (Q)  14	  2.8%
Glu (E)  38	  7.7%
Gly (G)  39	  7.9%
His (H)   8	  1.6%
Ile (I)  25	  5.1%
Leu (L)  42	  8.5%
Lys (K)  32	  6.5%
Met (M)   5	  1.0%
Phe (F)  16	  3.3%
Pro (P)  29	  5.9%
Ser (S)  25	  5.1%
Thr (T)  24	  4.9%
Trp (W)   9	  1.8%
Tyr (Y)  20	  4.1%
Val (V)  45	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2440
Hydrogen    H	      3815
Nitrogen    N	       647
Oxygen      O	       715
Sulfur      S	         9

Formula: C2440H3815N647O715S9
Total number of atoms: 7626

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    79550
Abs 0.1% (=1 g/l)   1.475, assuming all pairs of Cys residues form cystines


Ext. coefficient    79300
Abs 0.1% (=1 g/l)   1.470, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.65
This classifies the protein as stable.



Aliphatic index: 90.81

Grand average of hydropathicity (GRAVY): -0.164