ProtParam
User-provided sequence:
10 20 30 40 50 60
MSALFAVSRS AIALRSIAPR SSTATTSSFL ALARLYSSGK FPPHTVINMP ALSPTMTQGN
70 80 90 100 110 120
IQSWAKKVGD ELTPGEAIAE IETDKASMDF EFQEEGYLAK ILLDAGAKDV PVGQPIAVYV
130 140 150 160 170 180
EDAGEVAAFE NFTAADAGEA PKPAPAAEEE APKKEEPKAS TSTSAPASTP SPSSKKAPTD
190 200 210 220 230 240
RIIASPFAKT IALEKGISLK GIKGSGPNGR IVAKDLEGVE PQAAAAAAPA AAAATAGAAP
250 260 270 280 290 300
SATASYEDIP ITSMRKTIAS RLLQSTQQSP SYIIQSQISV SKLLKLRASL NATAEERYKL
310 320 330 340 350 360
SINDLLIKAI AKTCVRIPEV NAAWLGEQGV IRQYKNVDVS VAVATPTGLI TPIVTNAESK
370 380 390 400 410 420
GLAEISNQVK DLGKRAKVGK LLPEEFQGGT ICISNLGMNH AVTAFTSIIN PPQSAILAIG
430 440 450 460 470
TTEKKAVPSE VNEQGFVFDD VITITGTFDH RVIDGALGGE WMKELKRIVE NPLEMLI
References and
documentation are available.
Number of amino acids: 477
Molecular weight: 50062.22
Theoretical pI: 5.72
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 2219
Hydrogen H 3607
Nitrogen N 595
Oxygen O 695
Sulfur S 10
Formula: C
2219H
3607N
595O
695S
10
Total number of atoms: 7126
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27055
Abs 0.1% (=1 g/l) 0.540, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.538, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.74
This classifies the protein as unstable.
Aliphatic index: 91.59
Grand average of hydropathicity (GRAVY): -0.045