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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSALFAVSRS AIALRSIAPR SSTATTSSFL ALARLYSSGK FPPHTVINMP ALSPTMTQGN

70 80 90 100 110 120
IQSWAKKVGD ELTPGEAIAE IETDKASMDF EFQEEGYLAK ILLDAGAKDV PVGQPIAVYV

130 140 150 160 170 180
EDAGEVAAFE NFTAADAGEA PKPAPAAEEE APKKEEPKAS TSTSAPASTP SPSSKKAPTD

190 200 210 220 230 240
RIIASPFAKT IALEKGISLK GIKGSGPNGR IVAKDLEGVE PQAAAAAAPA AAAATAGAAP

250 260 270 280 290 300
SATASYEDIP ITSMRKTIAS RLLQSTQQSP SYIIQSQISV SKLLKLRASL NATAEERYKL

310 320 330 340 350 360
SINDLLIKAI AKTCVRIPEV NAAWLGEQGV IRQYKNVDVS VAVATPTGLI TPIVTNAESK

370 380 390 400 410 420
GLAEISNQVK DLGKRAKVGK LLPEEFQGGT ICISNLGMNH AVTAFTSIIN PPQSAILAIG

430 440 450 460 470
TTEKKAVPSE VNEQGFVFDD VITITGTFDH RVIDGALGGE WMKELKRIVE NPLEMLI


References and documentation are available.

Number of amino acids: 477 Molecular weight: 50062.22 Theoretical pI: 5.72
Amino acid composition: 
Ala (A) 71 14.9% Arg (R) 15 3.1% Asn (N) 15 3.1% Asp (D) 17 3.6% Cys (C) 2 0.4% Gln (Q) 16 3.4% Glu (E) 35 7.3% Gly (G) 31 6.5% His (H) 3 0.6% Ile (I) 39 8.2% Leu (L) 34 7.1% Lys (K) 33 6.9% Met (M) 8 1.7% Phe (F) 13 2.7% Pro (P) 32 6.7% Ser (S) 42 8.8% Thr (T) 33 6.9% Trp (W) 3 0.6% Tyr (Y) 7 1.5% Val (V) 28 5.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 52 Total number of positively charged residues (Arg + Lys): 48 Atomic composition: Carbon C 2219 Hydrogen H 3607 Nitrogen N 595 Oxygen O 695 Sulfur S 10 Formula: C2219H3607N595O695S10 Total number of atoms: 7126 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 27055 Abs 0.1% (=1 g/l) 0.540, assuming all pairs of Cys residues form cystines Ext. coefficient 26930 Abs 0.1% (=1 g/l) 0.538, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 42.74 This classifies the protein as unstable. Aliphatic index: 91.59 Grand average of hydropathicity (GRAVY): -0.045