ProtParam
User-provided sequence:
10 20 30 40 50 60
MSALRSTKGL VALVTGGASG LGKGAAEVLA KAGAQVAILD LPQSKGADVA KEIGGIFTPA
70 80 90 100 110 120
SVTSEEEVRA AFAKVQAEYG RLDALVNCAG IAYAFKLYSV QKKKHVDFEK IRQTIDVNVL
130 140 150 160 170 180
GTFNVIRHGV ALMGEHEKDA NGQRGVVINT ASVAAFDGQT GQSAYSASKG AIVGMTLPLA
190 200 210 220 230 240
RDFAGDGIRF NTIAPGLMDT PLLSSLPEKV KSFLAQLIPN PSRLGHPHEY GALVQHIIEN
250
QYLNGETIRF DGALRMPA
References and
documentation are available.
Number of amino acids: 258
Molecular weight: 27143.12
Theoretical pI: 8.58
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1207
Hydrogen H 1944
Nitrogen N 338
Oxygen O 360
Sulfur S 6
Formula: C
1207H
1944N
338O
360S
6
Total number of atoms: 3855
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.329, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.329, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 24.23
This classifies the protein as stable.
Aliphatic index: 96.12
Grand average of hydropathicity (GRAVY): 0.052