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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSALRSTKGL VALVTGGASG LGKGAAEVLA KAGAQVAILD LPQSKGADVA KEIGGIFTPA

70 80 90 100 110 120
SVTSEEEVRA AFAKVQAEYG RLDALVNCAG IAYAFKLYSV QKKKHVDFEK IRQTIDVNVL

130 140 150 160 170 180
GTFNVIRHGV ALMGEHEKDA NGQRGVVINT ASVAAFDGQT GQSAYSASKG AIVGMTLPLA

190 200 210 220 230 240
RDFAGDGIRF NTIAPGLMDT PLLSSLPEKV KSFLAQLIPN PSRLGHPHEY GALVQHIIEN

250
QYLNGETIRF DGALRMPA


References and documentation are available.

Number of amino acids: 258 Molecular weight: 27143.12 Theoretical pI: 8.58
Amino acid composition: 
Ala (A) 35 13.6% Arg (R) 11 4.3% Asn (N) 9 3.5% Asp (D) 11 4.3% Cys (C) 1 0.4% Gln (Q) 11 4.3% Glu (E) 13 5.0% Gly (G) 28 10.9% His (H) 6 2.3% Ile (I) 15 5.8% Leu (L) 24 9.3% Lys (K) 15 5.8% Met (M) 5 1.9% Phe (F) 10 3.9% Pro (P) 10 3.9% Ser (S) 15 5.8% Thr (T) 12 4.7% Trp (W) 0 0.0% Tyr (Y) 6 2.3% Val (V) 21 8.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 24 Total number of positively charged residues (Arg + Lys): 26 Atomic composition: Carbon C 1207 Hydrogen H 1944 Nitrogen N 338 Oxygen O 360 Sulfur S 6 Formula: C1207H1944N338O360S6 Total number of atoms: 3855 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 8940 Abs 0.1% (=1 g/l) 0.329, assuming all pairs of Cys residues form cystines Ext. coefficient 8940 Abs 0.1% (=1 g/l) 0.329, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 24.23 This classifies the protein as stable. Aliphatic index: 96.12 Grand average of hydropathicity (GRAVY): 0.052