ProtParam
User-provided sequence:
10 20 30 40 50 60
MSALYFKKLR KQQNPDVSSS EDSDSDEEIP KKKQKQQKPV AEEFDDFVVD RAVREEAPKE
70 80 90 100 110 120
GAFEAVDLLG TSYLEPYEMD KDLQELLEKS VVGPKFEGDH QPQNRLLGRN AAARLKKSER
130 140 150 160 170 180
EKTKGSAWFN LPATELTDEH KRDLEFLQMR STLDPLAHYR RNDRAVLPKY FQVGRVVDAP
190 200 210 220 230 240
EDFYSSRMVR KERKKTMVDE ILHNEESLSK AKKKYAEIRQ KEQSKRRGAF QKFGNRKSHK
250
QQRESKMKNK KK
References and
documentation are available.
Number of amino acids: 252
Molecular weight: 29551.45
Theoretical pI: 9.49
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 1293
Hydrogen H 2082
Nitrogen N 384
Oxygen O 397
Sulfur S 6
Formula: C
1293H
2082N
384O
397S
6
Total number of atoms: 4162
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.539
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.34
This classifies the protein as unstable.
Aliphatic index: 58.45
Grand average of hydropathicity (GRAVY): -1.245