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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSALYFKKLR KQQNPDVSSS EDSDSDEEIP KKKQKQQKPV AEEFDDFVVD RAVREEAPKE

70 80 90 100 110 120
GAFEAVDLLG TSYLEPYEMD KDLQELLEKS VVGPKFEGDH QPQNRLLGRN AAARLKKSER

130 140 150 160 170 180
EKTKGSAWFN LPATELTDEH KRDLEFLQMR STLDPLAHYR RNDRAVLPKY FQVGRVVDAP

190 200 210 220 230 240
EDFYSSRMVR KERKKTMVDE ILHNEESLSK AKKKYAEIRQ KEQSKRRGAF QKFGNRKSHK

250
QQRESKMKNK KK


References and documentation are available.

Number of amino acids: 252 Molecular weight: 29551.45 Theoretical pI: 9.49
Amino acid composition: 
Ala (A) 17 6.7% Arg (R) 21 8.3% Asn (N) 8 3.2% Asp (D) 18 7.1% Cys (C) 0 0.0% Gln (Q) 15 6.0% Glu (E) 27 10.7% Gly (G) 9 3.6% His (H) 5 2.0% Ile (I) 3 1.2% Leu (L) 20 7.9% Lys (K) 35 13.9% Met (M) 6 2.4% Phe (F) 11 4.4% Pro (P) 11 4.4% Ser (S) 18 7.1% Thr (T) 6 2.4% Trp (W) 1 0.4% Tyr (Y) 7 2.8% Val (V) 14 5.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 45 Total number of positively charged residues (Arg + Lys): 56 Atomic composition: Carbon C 1293 Hydrogen H 2082 Nitrogen N 384 Oxygen O 397 Sulfur S 6 Formula: C1293H2082N384O397S6 Total number of atoms: 4162 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15930 Abs 0.1% (=1 g/l) 0.539 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 59.34 This classifies the protein as unstable. Aliphatic index: 58.45 Grand average of hydropathicity (GRAVY): -1.245