ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSANDFKPET WTSSANEALR VSIVGENAVQ FSPLFTYPIY GDSEKIYGYK DLIIHLAFDS 

        70         80         90        100        110        120 
VTFKPYVNVK YSAKLGDDNI VDVEKKLLSF LPKDDVIVRD EAKWVDCFAE ERKTHNLSDV 

       130        140        150        160        170        180 
FEKVSEYSLN GEEFVVYKSS LVDDFARRMH RRVQIFSLLF IEAANYIDET DPSWQIYWLL 

       190        200        210        220        230        240 
NKKTKELIGF VTTYKYWHYL GAKSFDEDID KKFRAKISQF LIFPPYQNKG HGSCLYEAII 

       250        260        270        280        290        300 
QSWLEDKSIT EITVEDPNEA FDDLRDRNDI QRLRKLGYDA VFQKHSDLSD EFLESSRKSL 

       310        320        330        340        350        360 
KLEERQFNRL VEMLLLLNNS PSFELKVKNR LYIKNYDALD QTDPEKAREA LQNSFILVKD 

       370 
DYRRIIESIN KSQG 

References and documentation are available.

Number of amino acids: 374

Molecular weight: 43872.61

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  19	  5.1%
Arg (R)  19	  5.1%
Asn (N)  19	  5.1%
Asp (D)  33	  8.8%
Cys (C)   2	  0.5%
Gln (Q)  12	  3.2%
Glu (E)  30	  8.0%
Gly (G)  11	  2.9%
His (H)   6	  1.6%
Ile (I)  25	  6.7%
Leu (L)  37	  9.9%
Lys (K)  33	  8.8%
Met (M)   3	  0.8%
Phe (F)  23	  6.1%
Pro (P)  11	  2.9%
Ser (S)  31	  8.3%
Thr (T)  12	  3.2%
Trp (W)   6	  1.6%
Tyr (Y)  19	  5.1%
Val (V)  23	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      1993
Hydrogen    H	      3061
Nitrogen    N	       513
Oxygen      O	       594
Sulfur      S	         5

Formula: C1993H3061N513O594S5
Total number of atoms: 6166

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61435
Abs 0.1% (=1 g/l)   1.400, assuming all pairs of Cys residues form cystines


Ext. coefficient    61310
Abs 0.1% (=1 g/l)   1.397, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.56
This classifies the protein as stable.



Aliphatic index: 87.57

Grand average of hydropathicity (GRAVY): -0.505