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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSANNGVTGK LSSRVMNMKF MKFGKTDDEE SSNSNTPSNI NSDVEPIEQK GKLFGLDDSA

70 80 90 100 110 120
WDLNSYKDDL KKISGKEKKK VKRVVYKKRP NLIISNVGYS ELRKPEGVIS GRKTFGDNSD

130 140 150 160 170 180
DSGSRKRKFD EGEQNEDEKR DAKDKEFTGS QDDGEDEYDL DKLFKDSIKK KKTNHNGKNK


NRNSKK


References and documentation are available.

Number of amino acids: 186 Molecular weight: 21142.43 Theoretical pI: 9.29
Amino acid composition: 
Ala (A) 3 1.6% Arg (R) 9 4.8% Asn (N) 17 9.1% Asp (D) 21 11.3% Cys (C) 0 0.0% Gln (Q) 3 1.6% Glu (E) 14 7.5% Gly (G) 15 8.1% His (H) 1 0.5% Ile (I) 7 3.8% Leu (L) 9 4.8% Lys (K) 33 17.7% Met (M) 4 2.2% Phe (F) 7 3.8% Pro (P) 4 2.2% Ser (S) 20 10.8% Thr (T) 6 3.2% Trp (W) 1 0.5% Tyr (Y) 4 2.2% Val (V) 8 4.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 35 Total number of positively charged residues (Arg + Lys): 42 Atomic composition: Carbon C 904 Hydrogen H 1465 Nitrogen N 269 Oxygen O 307 Sulfur S 4 Formula: C904H1465N269O307S4 Total number of atoms: 2949 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 11460 Abs 0.1% (=1 g/l) 0.542 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.62 This classifies the protein as stable. Aliphatic index: 47.63 Grand average of hydropathicity (GRAVY): -1.461