ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSANSGVKRA SKAFKTCLFC KRSHVVCDKQ RPCSRCVKRD IAHLCREDDI AVPNEMPSQH 

        70         80         90        100        110        120 
ESSPNDNNIQ GKYANKAHTG IPSDYQNEPV NKSGSTYGEE LSPKLDSSLV NDTTSLLLPQ 

       130        140        150        160        170        180 
QPVFVSENVG SEFSSLNEFL SMLENPLLTQ TSLSSSSASN VHLENGSQTT QSPLEYQNDN 

       190        200        210        220        230        240 
RRDEIGVARQ ENRSPTIMSG SSNSISKGDK QDQEKEESRI LANANENSAP TPKEQFFLTA 

       250        260        270        280        290        300 
ADPSTEMTPE HRLKLVINAK LEAGLLKPYN YAKGYARLQD YMDKYMNQSS KQRILKPLST 

       310        320        330        340        350        360 
IRPAFRTIAR SLKDVDLVLV EESFERMLLS YDRVFTSMSM PACLCRRTGE IYRANKEFAS 

       370        380        390        400        410        420 
LVDCTVDDLR DGKLAIYELM TEESAVNFWE KYGSIAFDKG QKAVLTSCSL RTKDGIRKRP 

       430        440 
CCFSFTIRRD RYNIPICIVG NFIPLS 

References and documentation are available.

Number of amino acids: 446

Molecular weight: 50081.54

Theoretical pI: 7.87

Amino acid composition: 
Ala (A)  27	  6.1%
Arg (R)  29	  6.5%
Asn (N)  28	  6.3%
Asp (D)  25	  5.6%
Cys (C)  13	  2.9%
Gln (Q)  18	  4.0%
Glu (E)  31	  7.0%
Gly (G)  18	  4.0%
His (H)   6	  1.3%
Ile (I)  21	  4.7%
Leu (L)  39	  8.7%
Lys (K)  29	  6.5%
Met (M)  11	  2.5%
Phe (F)  16	  3.6%
Pro (P)  23	  5.2%
Ser (S)  52	 11.7%
Thr (T)  23	  5.2%
Trp (W)   1	  0.2%
Tyr (Y)  14	  3.1%
Val (V)  22	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2166
Hydrogen    H	      3467
Nitrogen    N	       621
Oxygen      O	       694
Sulfur      S	        24

Formula: C2166H3467N621O694S24
Total number of atoms: 6972

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27110
Abs 0.1% (=1 g/l)   0.541, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.526, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.52
This classifies the protein as unstable.



Aliphatic index: 72.83

Grand average of hydropathicity (GRAVY): -0.580