ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAPAQNYKI ADISLAAFGR KEIELAEHEM PGLMAIRKAY GDVQPLKGAR IAGCLHMTIQ 

        70         80         90        100        110        120 
TAVLIETLVA LGAEVTWSSC NIYSTQDHAA AAIAASGVPV FAWKGETEEE YLWCIEQQLF 

       130        140        150        160        170        180 
AFKDNKKLNL ILDDGGDLTT LVHEKHPEML EDCFGLSEET TTGVHHLYRM VKEGKLKVPA 

       190        200        210        220        230        240 
INVNDSVTKS KFDNLYGCRE SLVDGIKRAT DVMLAGKVAV VAGYGDVGKG CAAALRGMGA 

       250        260        270        280        290        300 
RVLVTEIDPI NALQAAMEGY QVVTMEDASH IGQVFVTTTG CRDIINGEHF INMPEDAIVC 

       310        320        330        340        350        360 
NIGHFDIEID VAWLKANAKE CINIKPQVDR YLLSSGRHVI LLANGRLVNL GCATGHSSFV 

       370        380        390        400        410        420 
MSCSFSNQVL AQIALFKSND KSFREKHIEF QKTGPFEVGV HVLPKILDEA VAKFHLGNLG 

       430        440 
VRLTKLSKVQ SEYLGIPEEG PFKADHYRY 

References and documentation are available.

Number of amino acids: 449

Molecular weight: 49125.51

Theoretical pI: 5.83

Amino acid composition: 
Ala (A)  45	 10.0%
Arg (R)  15	  3.3%
Asn (N)  18	  4.0%
Asp (D)  23	  5.1%
Cys (C)  11	  2.4%
Gln (Q)  14	  3.1%
Glu (E)  32	  7.1%
Gly (G)  37	  8.2%
His (H)  16	  3.6%
Ile (I)  30	  6.7%
Leu (L)  41	  9.1%
Lys (K)  29	  6.5%
Met (M)  12	  2.7%
Phe (F)  17	  3.8%
Pro (P)  13	  2.9%
Ser (S)  22	  4.9%
Thr (T)  21	  4.7%
Trp (W)   4	  0.9%
Tyr (Y)  12	  2.7%
Val (V)  37	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      2187
Hydrogen    H	      3463
Nitrogen    N	       591
Oxygen      O	       647
Sulfur      S	        23

Formula: C2187H3463N591O647S23
Total number of atoms: 6911

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40505
Abs 0.1% (=1 g/l)   0.825, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.812, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.83
This classifies the protein as stable.



Aliphatic index: 95.59

Grand average of hydropathicity (GRAVY): 0.004