ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS 

        70         80         90        100        110        120 
LPVKEFQIID TLLPGLQDEV MNIKPVQKQT RAGQRTRFKA VVVVGDSNGH VGLGIKTAKE 

       130        140        150        160        170        180 
VAGAIRAGII IAKLSVIPIR RGYWGTNLGQ PHSLATKTTG KCGSVTVRLI PAPRGSGIVA 

       190        200        210        220        230        240 
SPAVKKLLQL AGVEDVYTQS NGKTRTLENT LKAAFVAIGN TYGFLTPNLW AEQPLPVSPL 

       250 
DIYSDEASAQ KKRF 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 27449.81

Theoretical pI: 10.44

Amino acid composition: 
Ala (A)  21	  8.3%
Arg (R)  18	  7.1%
Asn (N)  10	  3.9%
Asp (D)   6	  2.4%
Cys (C)   1	  0.4%
Gln (Q)  13	  5.1%
Glu (E)  12	  4.7%
Gly (G)  28	 11.0%
His (H)   3	  1.2%
Ile (I)  17	  6.7%
Leu (L)  21	  8.3%
Lys (K)  20	  7.9%
Met (M)   2	  0.8%
Phe (F)   7	  2.8%
Pro (P)  16	  6.3%
Ser (S)  12	  4.7%
Thr (T)  19	  7.5%
Trp (W)   3	  1.2%
Tyr (Y)   4	  1.6%
Val (V)  21	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1224
Hydrogen    H	      2010
Nitrogen    N	       360
Oxygen      O	       349
Sulfur      S	         3

Formula: C1224H2010N360O349S3
Total number of atoms: 3946

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22460
Abs 0.1% (=1 g/l)   0.818, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   0.818, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.85
This classifies the protein as stable.



Aliphatic index: 90.59

Grand average of hydropathicity (GRAVY): -0.281