ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAPKSGGDP LPHPPKEQLP DISYCITSPP PWPEAVLLGF QHYLVMLGTT VLIPSALVPQ 

        70         80         90        100        110        120 
MGGRNEEKAK LIQTILFVAG LNTLLQTVFG TRLPAVIGAS YTFVPVTISI MLSGRFNDVA 

       130        140        150        160        170        180 
DPVERFKRII RATQGALIVA STLQIILGFS GLWRNVVRFL SPLSAAPLVG LVGYGLYELG 

       190        200        210        220        230        240 
FPGVAKCIEI GLPGLIILIL ISQYMPHVIK GGKHVFARFA VIFSVAIVWL YAFFLTLGGA 

       250        260        270        280        290        300 
YNGVGTDTQR SCRTDRAGLI SAAPWIRVPW PFQWGAPLFD AGEAFAMMMA SFVALVESTG 

       310        320        330        340        350        360 
AFIAVSRYAS ATMPPPSVIS RGVGWQGVAI LISGLFGTGI GSSVSVENAG LLALTKIGSR 

       370        380        390        400        410        420 
RVVQISAGFM IFFSILGKFG AVFASIPSPI IAALYCLFFA YVGAGGLSLL QFCNLNSFRT 

       430        440        450        460        470        480 
LFILGFSIFL GLSIPQYFNE HTAIKGYGPV HTGARWFNDM VNVPFSSKAF VGGCVAYLLD 

       490        500        510        520 
TTLHKKDGSI RKDRGKHWWD RFWTFKNDPR TEEFYALPFN LNKYFPSV 

References and documentation are available.

Number of amino acids: 528

Molecular weight: 57321.28

Theoretical pI: 9.57

Amino acid composition: 
Ala (A)  47	  8.9%
Arg (R)  23	  4.4%
Asn (N)  14	  2.7%
Asp (D)  13	  2.5%
Cys (C)   6	  1.1%
Gln (Q)  14	  2.7%
Glu (E)  13	  2.5%
Gly (G)  54	 10.2%
His (H)   8	  1.5%
Ile (I)  41	  7.8%
Leu (L)  56	 10.6%
Lys (K)  18	  3.4%
Met (M)  11	  2.1%
Phe (F)  41	  7.8%
Pro (P)  35	  6.6%
Ser (S)  39	  7.4%
Thr (T)  26	  4.9%
Trp (W)  11	  2.1%
Tyr (Y)  16	  3.0%
Val (V)  42	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      2681
Hydrogen    H	      4118
Nitrogen    N	       670
Oxygen      O	       690
Sulfur      S	        17

Formula: C2681H4118N670O690S17
Total number of atoms: 8176

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    84715
Abs 0.1% (=1 g/l)   1.478, assuming all pairs of Cys residues form cystines


Ext. coefficient    84340
Abs 0.1% (=1 g/l)   1.471, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.52
This classifies the protein as stable.



Aliphatic index: 103.62

Grand average of hydropathicity (GRAVY): 0.498