ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAPSPFIKK LAANDRKTRD KALESLQRFL SQKKKFERLD FLKLWKGLFY CMWMADKPLY 

        70         80         90        100        110        120 
QQKLSDNLAA LVPIVWIDNR ILFQSTFWET MGREWTGIDI LRTDKFYLLM RRFCAAAFRD 

       130        140        150        160        170        180 
IQTRSKTALL DKVVAEYNQM WMDGPFNTEN LAFPNGILFH LADIWTEELR KVYPEDVPKA 

       190        200        210 
DWYLPFDSTI KSSHNVVLRK TLPKRLDRVS EYTKDSQ 

References and documentation are available.

Number of amino acids: 217

Molecular weight: 25747.90

Theoretical pI: 9.41

Amino acid composition: 
Ala (A)  15	  6.9%
Arg (R)  15	  6.9%
Asn (N)   8	  3.7%
Asp (D)  17	  7.8%
Cys (C)   2	  0.9%
Gln (Q)   8	  3.7%
Glu (E)  10	  4.6%
Gly (G)   5	  2.3%
His (H)   2	  0.9%
Ile (I)  10	  4.6%
Leu (L)  26	 12.0%
Lys (K)  20	  9.2%
Met (M)   7	  3.2%
Phe (F)  14	  6.5%
Pro (P)  10	  4.6%
Ser (S)  12	  5.5%
Thr (T)  12	  5.5%
Trp (W)   8	  3.7%
Tyr (Y)   7	  3.2%
Val (V)   9	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1180
Hydrogen    H	      1826
Nitrogen    N	       310
Oxygen      O	       319
Sulfur      S	         9

Formula: C1180H1826N310O319S9
Total number of atoms: 3644

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54555
Abs 0.1% (=1 g/l)   2.119, assuming all pairs of Cys residues form cystines


Ext. coefficient    54430
Abs 0.1% (=1 g/l)   2.114, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.30
This classifies the protein as unstable.



Aliphatic index: 83.64

Grand average of hydropathicity (GRAVY): -0.408