ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAPVPQLVN ISHALQASTI QQIRLDMVDF NKDCKLSSIQ LARIDKYIDS LQAALNQFTK 

        70         80         90        100        110        120 
DNLHIERKEK NVTEADIQLY SGLKSMYLDY LNQLIKLKHE KQHHSTPPIA NDVSLDFFVN 

       130        140        150        160        170        180 
QLPKFSPEER KNYIDNLILN KNSHNRLSKM DGLVDAVINL CVLDTSVAEN VRSYMKLLDT 

       190        200        210        220        230        240 
LGFQKGSNST GTKANLKKKL ASSKAKIKDS EKEKEKEKDK SKVKMKTKLK PSPLLNNDDK 

       250        260        270        280        290        300 
NSSPSPTAST SSMKKLKSGL FNKNEAKSTE SLPTSSKKKL SFSKYLNKDD ADMTKLGTKR 

       310        320        330        340        350        360 
SIDVDFKVNP EASTVASNII SSSTSGSSTT TVATPASSEE PLKKKTKISV QDSNVQSILR 

       370        380        390        400        410        420 
NGKPKKARIS SIKFLDDSQL IKVYGDDLPN QGLQVSPTQL KKILKPFKEG EPKEIILFED 

       430        440        450        460        470        480 
MSIKLKPLDL MFLKNTNSDD YMDISETKGG PIHCETRTPL IYRKNFNHFN PDLNKRPPRE 

       490        500        510        520        530 
PIEFDLNGNT NSTPTIAKAF GKNSLLLRKD RGGLPYKHVP IVKRNKYPPR PVH 

References and documentation are available.

Number of amino acids: 533

Molecular weight: 59818.67

Theoretical pI: 9.63

Amino acid composition: 
Ala (A)  23	  4.3%
Arg (R)  17	  3.2%
Asn (N)  39	  7.3%
Asp (D)  37	  6.9%
Cys (C)   3	  0.6%
Gln (Q)  18	  3.4%
Glu (E)  24	  4.5%
Gly (G)  18	  3.4%
His (H)  10	  1.9%
Ile (I)  32	  6.0%
Leu (L)  56	 10.5%
Lys (K)  73	 13.7%
Met (M)  11	  2.1%
Phe (F)  17	  3.2%
Pro (P)  32	  6.0%
Ser (S)  58	 10.9%
Thr (T)  30	  5.6%
Trp (W)   0	  0.0%
Tyr (Y)  12	  2.3%
Val (V)  23	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      2641
Hydrogen    H	      4326
Nitrogen    N	       734
Oxygen      O	       813
Sulfur      S	        14

Formula: C2641H4326N734O813S14
Total number of atoms: 8528

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18005
Abs 0.1% (=1 g/l)   0.301, assuming all pairs of Cys residues form cystines


Ext. coefficient    17880
Abs 0.1% (=1 g/l)   0.299, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.59
This classifies the protein as unstable.



Aliphatic index: 81.22

Grand average of hydropathicity (GRAVY): -0.707