ProtParam
User-provided sequence:
10 20 30 40 50 60
MSARRVTLNT RVSRASTSTP VGGASTSSRV GATSPTSPTR TSRQQEKEEL QHLNDRLACY
70 80 90 100 110 120
IDRMRNLENE NSRLTQELNL AQDTVNRETS NLKAVYEKEL AAARKLLDET AKEKAKLEID
130 140 150 160 170 180
IKRLWEENDD LKPRLDKKTK EATVAENNAR LYENRYNEVN GKYNQSLADR KKFEDQAKEL
190 200 210 220 230 240
ALENERLRRQ LDDLRKQLEA ETLARVDLEN QNQSLREELA FKDQVHTQEL TETRSRRQIE
250 260 270 280 290 300
ISEIDGRLSR QYEAKLQQSL QELRDQYEGQ MRINREEIEL LYDNEIQNLK AAANRAAQGS
310 320 330 340 350 360
ALATEEVRLM RTKIDGLNAK LQNLEDTNAG LNARIRELEN LLDTERQRHN QYIASLEAEL
370 380 390 400 410 420
QRMRDEMAHQ LQEYQGLMDI KVSLDLEIAA YDKLLCGEER RLNIESPGRP TTDSGISSNG
430 440 450 460 470 480
SHLTASASSR SGRVTPSGRR SATPGISGSS AVKRRRTVID ESEDRTLSEY SVNAAAKGDL
490 500 510 520 530 540
EIIEADVEGR FIKLHNKGTE EINLTGWQLT RIAGDEELAF KFSRGSKVLG GASVTIWSVD
550 560 570 580 590 600
AGTAHDPPNN LVMKKKWPVA NSMRSVLANA DKEDVASYDR VRANVSSHTS RHRSSGTPST
610 620
GFTLGSGAGS TGVRSLFSLL F
References and
documentation are available.
Number of amino acids: 621
Molecular weight: 69859.73
Theoretical pI: 6.42
Total number of negatively charged residues (Asp + Glu): 98
Total number of positively charged residues (Arg + Lys): 95
Atomic composition:
Carbon C 2971
Hydrogen H 4882
Nitrogen N 928
Oxygen O 994
Sulfur S 11
Formula: C
2971H
4882N
928O
994S
11
Total number of atoms: 9786
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41495
Abs 0.1% (=1 g/l) 0.594, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 0.592, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.17
This classifies the protein as unstable.
Aliphatic index: 79.40
Grand average of hydropathicity (GRAVY): -0.832