ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSASKEEIAA LIVNYFSSIV EKKEISEDGA DSLNVAMDCI SEAFGFEREA VSGILGKSEF 

        70         80         90        100        110        120 
KGQHLADILN SASRVPESNK KDDAENVEIN IPEDDAETKA KAEDLKMQGN KAMANKDYEL 

       130        140        150        160        170        180 
AINKYTEAIK VLPTNAIYYA NRAAAHSSLK EYDQAVKDAE SAISIDPSYF RGYSRLGFAK 

       190        200        210        220        230        240 
YAQGKPEEAL EAYKKVLDIE GDNATEAMKR DYESAKKKVE QSLNLEKTVP EQSRDADVDA 

       250        260        270        280        290        300 
SQGASAGGLP DLGSLLGGGL GGLMNNPQLM QAAQKMMSNP GAMQNIQKMM QDPSIRQMAE 

       310        320        330        340 
GFASGGGTPN LSDLMNNPAL RNMAGNLFGG AGAQSTDETP DNENKQ 

References and documentation are available.

Number of amino acids: 346

Molecular weight: 37218.44

Theoretical pI: 4.68

Amino acid composition: 
Ala (A)  45	 13.0%
Arg (R)   9	  2.6%
Asn (N)  24	  6.9%
Asp (D)  24	  6.9%
Cys (C)   1	  0.3%
Gln (Q)  16	  4.6%
Glu (E)  31	  9.0%
Gly (G)  28	  8.1%
His (H)   2	  0.6%
Ile (I)  17	  4.9%
Leu (L)  25	  7.2%
Lys (K)  28	  8.1%
Met (M)  15	  4.3%
Phe (F)   8	  2.3%
Pro (P)  13	  3.8%
Ser (S)  29	  8.4%
Thr (T)   8	  2.3%
Trp (W)   0	  0.0%
Tyr (Y)  11	  3.2%
Val (V)  12	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1597
Hydrogen    H	      2551
Nitrogen    N	       445
Oxygen      O	       545
Sulfur      S	        16

Formula: C1597H2551N445O545S16
Total number of atoms: 5154

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16390
Abs 0.1% (=1 g/l)   0.440, assuming all pairs of Cys residues form cystines


Ext. coefficient    16390
Abs 0.1% (=1 g/l)   0.440, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.80
This classifies the protein as unstable.



Aliphatic index: 70.40

Grand average of hydropathicity (GRAVY): -0.599