ProtParam
User-provided sequence:
10 20 30 40 50 60
MSASLVRATV RAVSKRKLQP TRAALTLTPS AVNKIKQLLK DKPEHVGLKV GVRTRGCNGL
70 80 90 100 110 120
SYSLEYTKTK GDSDEEVIQD GVRVFIEKKA QLTLLGTEMD YVEDKLSSEF VFNNPNIKGT
CGCGESFHV
References and
documentation are available.
Number of amino acids: 129
Molecular weight: 14188.33
Theoretical pI: 9.19
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 621
Hydrogen H 1024
Nitrogen N 176
Oxygen O 192
Sulfur S 5
Formula: C
621H
1024N
176O
192S
5
Total number of atoms: 2018
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4595
Abs 0.1% (=1 g/l) 0.324, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.315, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.19
This classifies the protein as stable.
Aliphatic index: 86.05
Grand average of hydropathicity (GRAVY): -0.347