ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSASSSSFEQ PQVITCNAAV AWRAGEPLVM EEVEVSPPQP LEIRIKVVCT SLCRSDLSAW 

        70         80         90        100        110        120 
ESQSLLPRIF GHEAAGIVES IGEGVTEFEK GDHVLAVFTG ECGSCRHCIS GKSNMCQVLG 

       130        140        150        160        170        180 
MERKGLMHSD QKTRFSIKGK PVYHYCAVSS FSEYTVVHSG CAVKVDPLAP LHKICLLSCG 

       190        200        210        220        230        240 
VAAGLGAAWN VADVQKGSSV VIFGLGTVGL SVAQGAKLRG AAQILGVDIN PAKAEQAKTF 

       250        260        270        280        290        300 
GVTDFINSND LSEPIPQVIK RMTGGGADFS FECVGDTGIA TTALQSCSDG WGMTVTLGVP 

       310        320        330        340        350        360 
KAKPEVSAHY GLFLSGKSLK GTLFGGWKPK SDLPSLIDKY MNKEIMIDEF ITHNLSFDEI 

       370        380 
NKAFVLMREG KCLRCVLHMP K 

References and documentation are available.

Number of amino acids: 381

Molecular weight: 40724.02

Theoretical pI: 6.55

Amino acid composition: 
Ala (A)  30	  7.9%
Arg (R)  11	  2.9%
Asn (N)   9	  2.4%
Asp (D)  15	  3.9%
Cys (C)  15	  3.9%
Gln (Q)  12	  3.1%
Glu (E)  23	  6.0%
Gly (G)  38	 10.0%
His (H)  10	  2.6%
Ile (I)  21	  5.5%
Leu (L)  31	  8.1%
Lys (K)  25	  6.6%
Met (M)  11	  2.9%
Phe (F)  16	  4.2%
Pro (P)  17	  4.5%
Ser (S)  36	  9.4%
Thr (T)  17	  4.5%
Trp (W)   5	  1.3%
Tyr (Y)   5	  1.3%
Val (V)  34	  8.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1800
Hydrogen    H	      2863
Nitrogen    N	       485
Oxygen      O	       537
Sulfur      S	        26

Formula: C1800H2863N485O537S26
Total number of atoms: 5711

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35825
Abs 0.1% (=1 g/l)   0.880, assuming all pairs of Cys residues form cystines


Ext. coefficient    34950
Abs 0.1% (=1 g/l)   0.858, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.38
This classifies the protein as unstable.



Aliphatic index: 86.98

Grand average of hydropathicity (GRAVY): 0.086