ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSASWADVAD SENTGSGSSN QNSHPSRPAY VPPHLRNRPA ASEPVAPLPA NDRVGYGGPP 

        70         80         90        100        110        120 
SGSRWAPGGS GVGVGGGGGY RADAGRPGSG SGYGGRGGGG WNNRSGGWDR REREVNPFEN 

       130        140        150        160        170        180 
DDSEPEPAFT EQDNTVINFD AYEDIPIETS GDNVPPPVNT FAEIDLGEAL NLNIRRCKYV 

       190        200        210        220        230        240 
KPTPVQRHAI PILLEGRDLM ACAQTGSGKT AAFCFPIISG IMKDQHVQRP RGSRTVYPLA 

       250        260        270        280        290        300 
VILSPTRELA SQIHDEAKKF SYQTGVKVVV AYGGTPINQQ LRELERGVDI LVATPGRLND 

       310        320        330        340        350        360 
LLERARVSMQ MIRFLALDEA DRMLDMGFEP QIRKIVEQMD MPPRGVRQTL LFSATFPREI 

       370        380        390        400        410        420 
QRLAADFLAN YIFLAVGRVG SSTDLIVQRV EFVLDSDKRS HLMDLLHAQR ENGIQGKQAL 

       430        440        450        460        470        480 
TLVFVETKRG ADSLENWLCI NGFPATSIHG DRTQQEREVA LKAFKSGRTP ILVATDVAAR 

       490        500        510        520        530        540 
GLDIPHVAHV VNFDLPNDID DYVHRIGRTG RAGKSGLATA FFNDGNTSLA RPLAELMQEA 

       550        560        570        580        590        600 
NQEVPEWLTR YASRSSFGGG KNRRSGGRFG GRDFRREGSF GSGRGGYGGG GGGYGGGGGY 

       610        620        630 
GGGGGYGGGG GYGGGYGGAS SGGYGGEPPS AWD 

References and documentation are available.

Number of amino acids: 633

Molecular weight: 67626.29

Theoretical pI: 6.63

Amino acid composition: 
Ala (A)  52	  8.2%
Arg (R)  54	  8.5%
Asn (N)  27	  4.3%
Asp (D)  37	  5.8%
Cys (C)   4	  0.6%
Gln (Q)  23	  3.6%
Glu (E)  34	  5.4%
Gly (G)  97	 15.3%
His (H)  11	  1.7%
Ile (I)  27	  4.3%
Leu (L)  43	  6.8%
Lys (K)  15	  2.4%
Met (M)  11	  1.7%
Phe (F)  24	  3.8%
Pro (P)  40	  6.3%
Ser (S)  43	  6.8%
Thr (T)  26	  4.1%
Trp (W)   7	  1.1%
Tyr (Y)  19	  3.0%
Val (V)  39	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2950
Hydrogen    H	      4601
Nitrogen    N	       889
Oxygen      O	       914
Sulfur      S	        15

Formula: C2950H4601N889O914S15
Total number of atoms: 9369

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67060
Abs 0.1% (=1 g/l)   0.992, assuming all pairs of Cys residues form cystines


Ext. coefficient    66810
Abs 0.1% (=1 g/l)   0.988, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.93
This classifies the protein as unstable.



Aliphatic index: 69.21

Grand average of hydropathicity (GRAVY): -0.484