ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAVEISPDV LVYKSPLTEQ STEYASISNN SDQTIAFKVK TTAPKFYCVR PNAAVVAPGE 

        70         80         90        100        110        120 
TIQVQVIFLG LTEEPAADFK CRDKFLVITL PSPYDLNGKA VADVWSDLEA EFKQQAISKK 

       130        140        150        160        170        180 
IKVKYLISPD VHPAQNQNIQ ENKETVEPVV QDSEPKEVPA VVNEKEVPAE PETQPPVQVK 

       190        200        210        220        230        240 
KEEVPPVVQK TVPHENEKQT SNSTPAPQNQ IKEAATVPAE NESSSMGIFI LVALLILVLG 


WFYR 

References and documentation are available.

Number of amino acids: 244

Molecular weight: 26925.65

Theoretical pI: 4.85

Amino acid composition: 
Ala (A)  21	  8.6%
Arg (R)   3	  1.2%
Asn (N)  12	  4.9%
Asp (D)   9	  3.7%
Cys (C)   2	  0.8%
Gln (Q)  16	  6.6%
Glu (E)  24	  9.8%
Gly (G)   5	  2.0%
His (H)   2	  0.8%
Ile (I)  14	  5.7%
Leu (L)  14	  5.7%
Lys (K)  20	  8.2%
Met (M)   2	  0.8%
Phe (F)   8	  3.3%
Pro (P)  23	  9.4%
Ser (S)  17	  7.0%
Thr (T)  14	  5.7%
Trp (W)   2	  0.8%
Tyr (Y)   6	  2.5%
Val (V)  30	 12.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1211
Hydrogen    H	      1921
Nitrogen    N	       307
Oxygen      O	       376
Sulfur      S	         4

Formula: C1211H1921N307O376S4
Total number of atoms: 3819

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20065
Abs 0.1% (=1 g/l)   0.745, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.10
This classifies the protein as unstable.



Aliphatic index: 89.02

Grand average of hydropathicity (GRAVY): -0.295