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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAVFNNATL SGLVQASTYS QTLQNVAHYQ PQLNFMEKYW AAWYSYMNND VLATGLMFFL

70 80 90 100 110 120
LHEFMYFFRC LPWFIIDQIP YFRRWKLQPT KIPSAKEQLY CLKSVLLSHF LVEAIPIWTF

130 140 150 160 170 180
HPMCEKLGIT VEVPFPSLKT MALEIGLFFV LEDTWHYWAH RLFHYGVFYK YIHKQHHRYA

190 200 210 220 230 240
APFGLSAEYA HPAETLSLGF GTVGMPILYV MYTGKLHLFT LCVWITLRLF QAVDSHSGYD

250 260 270 280 290 300
FPWSLNKIMP FWAGAEHHDL HHHYFIGNYA SSFRWWDYCL DTESGPEAKA SREERMKKRA


ENNAQKKTN


References and documentation are available.

Number of amino acids: 309 Molecular weight: 36479.13 Theoretical pI: 8.17
Amino acid composition: 
Ala (A) 24 7.8% Arg (R) 10 3.2% Asn (N) 11 3.6% Asp (D) 8 2.6% Cys (C) 5 1.6% Gln (Q) 11 3.6% Glu (E) 16 5.2% Gly (G) 14 4.5% His (H) 18 5.8% Ile (I) 13 4.2% Leu (L) 33 10.7% Lys (K) 16 5.2% Met (M) 11 3.6% Phe (F) 24 7.8% Pro (P) 15 4.9% Ser (S) 18 5.8% Thr (T) 16 5.2% Trp (W) 12 3.9% Tyr (Y) 20 6.5% Val (V) 14 4.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 24 Total number of positively charged residues (Arg + Lys): 26 Atomic composition: Carbon C 1712 Hydrogen H 2487 Nitrogen N 425 Oxygen O 434 Sulfur S 16 Formula: C1712H2487N425O434S16 Total number of atoms: 5074 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 96050 Abs 0.1% (=1 g/l) 2.633, assuming all pairs of Cys residues form cystines Ext. coefficient 95800 Abs 0.1% (=1 g/l) 2.626, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 45.54 This classifies the protein as unstable. Aliphatic index: 78.96 Grand average of hydropathicity (GRAVY): -0.102