ProtParam
User-provided sequence:
10 20 30 40 50 60
MSAVFNNATL SGLVQASTYS QTLQNVAHYQ PQLNFMEKYW AAWYSYMNND VLATGLMFFL
70 80 90 100 110 120
LHEFMYFFRC LPWFIIDQIP YFRRWKLQPT KIPSAKEQLY CLKSVLLSHF LVEAIPIWTF
130 140 150 160 170 180
HPMCEKLGIT VEVPFPSLKT MALEIGLFFV LEDTWHYWAH RLFHYGVFYK YIHKQHHRYA
190 200 210 220 230 240
APFGLSAEYA HPAETLSLGF GTVGMPILYV MYTGKLHLFT LCVWITLRLF QAVDSHSGYD
250 260 270 280 290 300
FPWSLNKIMP FWAGAEHHDL HHHYFIGNYA SSFRWWDYCL DTESGPEAKA SREERMKKRA
ENNAQKKTN
References and
documentation are available.
Number of amino acids: 309
Molecular weight: 36479.13
Theoretical pI: 8.17
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1712
Hydrogen H 2487
Nitrogen N 425
Oxygen O 434
Sulfur S 16
Formula: C
1712H
2487N
425O
434S
16
Total number of atoms: 5074
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 96050
Abs 0.1% (=1 g/l) 2.633, assuming all pairs of Cys residues form cystines
Ext. coefficient 95800
Abs 0.1% (=1 g/l) 2.626, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.54
This classifies the protein as unstable.
Aliphatic index: 78.96
Grand average of hydropathicity (GRAVY): -0.102