ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAWRKAGIS YAAYLNVAAQ AIRSSLKTEL QTASVLNRSQ TDAFYTQYKN GTAASEPTPI 


TK 

References and documentation are available.

Number of amino acids: 62

Molecular weight: 6742.58

Theoretical pI: 9.77

Amino acid composition: 
Ala (A)  11	 17.7%
Arg (R)   3	  4.8%
Asn (N)   3	  4.8%
Asp (D)   1	  1.6%
Cys (C)   0	  0.0%
Gln (Q)   4	  6.5%
Glu (E)   2	  3.2%
Gly (G)   2	  3.2%
His (H)   0	  0.0%
Ile (I)   3	  4.8%
Leu (L)   4	  6.5%
Lys (K)   4	  6.5%
Met (M)   1	  1.6%
Phe (F)   1	  1.6%
Pro (P)   2	  3.2%
Ser (S)   7	 11.3%
Thr (T)   7	 11.3%
Trp (W)   1	  1.6%
Tyr (Y)   4	  6.5%
Val (V)   2	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 3
Total number of positively charged residues (Arg + Lys): 7

Atomic composition:

Carbon      C	       297
Hydrogen    H	       473
Nitrogen    N	        83
Oxygen      O	        94
Sulfur      S	         1

Formula: C297H473N83O94S1
Total number of atoms: 948

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11460
Abs 0.1% (=1 g/l)   1.700

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.63
This classifies the protein as unstable.



Aliphatic index: 71.13

Grand average of hydropathicity (GRAVY): -0.373