ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSAYIAMKGV ITNVDENIRN DEDVAFEYEI QKTPQNILTW KRYIEYWKEE GRTDKQIRWL 

        70         80         90        100        110        120 
YERFCSQFVT DTSIWEDYIR WESTKEVVET SRIFWLFQRC LKSCVRDCDR ICLSYLELAI 

       130        140        150        160        170        180 
EQYDLAMIRH ALASSLMKME REMHRKVWDP VIKFVEEKVL PLTQLDSTQE DEEESTDEAE 

       190        200        210        220        230        240 
LINVLLVKGF TKGGFISEEI SENGSRGDIW SSHILERYLK VAPQQKRNES LATLALTRDN 

       250        260        270        280        290        300 
ITIKSVYEKY LPQDENSGKY LPSSELPFEL NFNYLASLEK LGLDNQYEEF MRQMNGIYPD 

       310        320        330        340        350        360 
KWLFLILSLA KYYISRGRLD SCGDLLKKSL QQTLRYSDFD RIYNFYLLFE QECSQFILGK 

       370        380        390        400        410        420 
LKENDSKFFN QKDWTEKLQA HMATFESLIN LYDIYLNDVA LRQDSNLVET WMKRVSLQKS 

       430        440        450        460        470        480 
AAEKCNVYSE AILKIDPRKV GTPGSFGRLW CSYGDLYWRS NAISTARELW TQSLKVPYPY 

       490        500        510        520        530        540 
IEDLEEIYLN WADRELDKEG VERAFSILED ALHVPTNPEI LLEKYKNGHR KIPAQTVLFN 

       550        560        570        580        590        600 
SLRIWSKYID YLEAYCPKDA NSSDKIFNKT KMAYNTVIDL RLITPAMAEN FALFLQNHYE 

       610        620        630        640        650        660 
VMESFQVYEK TIPLFPPEIQ YELWIEYLEV ATSHQLSSLS PEHIRFLFEK ALKNLCSNGI 

       670        680        690        700        710        720 
DCKTIFIAYS VFEERISGLI SKSIEILRRG AVIGTVSVST HLESRLQLWR MCISKAESTL 

       730        740        750        760        770        780 
GPSVTRELYQ ECIQILPNSK AVEFVIKFSD FESSIGETIR AREILAYGAK LLPPSRNTEL 

       790        800        810        820        830        840 
WDSFEIFELK HGDKETYKDM LKMKKVLESN MLIDSASVSH EEGNINFVAA ATSHAPNSHT 

       850 
LTQSTSSYSI NPDEIELDI 

References and documentation are available.

Number of amino acids: 859

Molecular weight: 100229.19

Theoretical pI: 5.17

Amino acid composition: 
Ala (A)  45	  5.2%
Arg (R)  43	  5.0%
Asn (N)  39	  4.5%
Asp (D)  45	  5.2%
Cys (C)  14	  1.6%
Gln (Q)  31	  3.6%
Glu (E)  85	  9.9%
Gly (G)  28	  3.3%
His (H)  14	  1.6%
Ile (I)  66	  7.7%
Leu (L)  94	 10.9%
Lys (K)  58	  6.8%
Met (M)  17	  2.0%
Phe (F)  37	  4.3%
Pro (P)  27	  3.1%
Ser (S)  76	  8.8%
Thr (T)  41	  4.8%
Trp (W)  19	  2.2%
Tyr (Y)  42	  4.9%
Val (V)  38	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 130
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      4521
Hydrogen    H	      7005
Nitrogen    N	      1163
Oxygen      O	      1349
Sulfur      S	        31

Formula: C4521H7005N1163O1349S31
Total number of atoms: 14069

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   167955
Abs 0.1% (=1 g/l)   1.676, assuming all pairs of Cys residues form cystines


Ext. coefficient   167080
Abs 0.1% (=1 g/l)   1.667, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.93
This classifies the protein as unstable.



Aliphatic index: 90.71

Grand average of hydropathicity (GRAVY): -0.366