ProtParam
User-provided sequence:
10 20 30 40 50 60
MSCLVLPLVS VGNIPQLSID WLLNSQANEW EYLEALDSKY LVEFVGPLDR PEDGSDSLYK
70 80 90 100 110 120
DADMKYSSAL EVFYNKKRGL FAIQQRTPLV SVNYLNNFIV EIILPFLSKY NISEICIWDS
130 140 150 160 170 180
LYAMEDENGV IVRPQEVYSL GEFYFDDEAE LLSNLHLNDQ ESMVNNWLHF TPTSFQDKIS
190 200 210 220 230 240
VDQPIFKILF QILNASQRPK ALRSIKYCSC LANEGDNSLD SQQFLQWIIS QKVIKNAPPI
250 260
VKFVRPISWQ GAYGMADARD KFVDLYN
References and
documentation are available.
Number of amino acids: 267
Molecular weight: 30739.03
Theoretical pI: 4.59
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1401
Hydrogen H 2140
Nitrogen N 348
Oxygen O 412
Sulfur S 9
Formula: C
1401H
2140N
348O
412S
9
Total number of atoms: 4310
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52620
Abs 0.1% (=1 g/l) 1.712, assuming all pairs of Cys residues form cystines
Ext. coefficient 52370
Abs 0.1% (=1 g/l) 1.704, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.33
This classifies the protein as unstable.
Aliphatic index: 99.29
Grand average of hydropathicity (GRAVY): -0.137