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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSCVYAFGSN GQRQLGLGHD EDMDTPQRSV PGDDGAIVRK IACGGNHSVM LTNDGNLVGC

70 80 90 100 110 120
GDNRRGELDS AQALRQVHDW RPVEVPAPVV DVACGWDTTV IVDADGRVWQ RGGGCYEFTQ

130 140 150 160 170 180
QHVPLNSNDE RIAVYGCFQN FVVVQGTRVY GWGSNTKCQL QEPKSRSLKE PVLVYDTGSV

190 200 210 220 230 240
AVDYVAMGKD FMVIVDEGGR IVHASGRLPT GFELKQQQKR HNLVVLCMWT SIHLWNARLN

250 260 270 280 290 300
TVESFGRGTH SQLFPQERLD FPIVGVATGS EHGILTTANQ EGKSHCYNVY CWGWGEHGNC

310 320 330
GPQKASQPGL QLVGQYSGKP RVFGGCATTW IVL


References and documentation are available.

Number of amino acids: 333 Molecular weight: 36481.05 Theoretical pI: 6.25
Amino acid composition: 
Ala (A) 17 5.1% Arg (R) 19 5.7% Asn (N) 15 4.5% Asp (D) 19 5.7% Cys (C) 12 3.6% Gln (Q) 22 6.6% Glu (E) 14 4.2% Gly (G) 42 12.6% His (H) 11 3.3% Ile (I) 10 3.0% Leu (L) 22 6.6% Lys (K) 10 3.0% Met (M) 6 1.8% Phe (F) 10 3.0% Pro (P) 14 4.2% Ser (S) 18 5.4% Thr (T) 17 5.1% Trp (W) 9 2.7% Tyr (Y) 9 2.7% Val (V) 37 11.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 33 Total number of positively charged residues (Arg + Lys): 29 Atomic composition: Carbon C 1596 Hydrogen H 2464 Nitrogen N 468 Oxygen O 481 Sulfur S 18 Formula: C1596H2464N468O481S18 Total number of atoms: 5027 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 63660 Abs 0.1% (=1 g/l) 1.745, assuming all pairs of Cys residues form cystines Ext. coefficient 62910 Abs 0.1% (=1 g/l) 1.724, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 31.97 This classifies the protein as stable. Aliphatic index: 74.80 Grand average of hydropathicity (GRAVY): -0.318