ProtParam
User-provided sequence:
10 20 30 40 50 60
MSCVYAFGSN GQRQLGLGHD EDMDTPQRSV PGDDGAIVRK IACGGNHSVM LTNDGNLVGC
70 80 90 100 110 120
GDNRRGELDS AQALRQVHDW RPVEVPAPVV DVACGWDTTV IVDADGRVWQ RGGGCYEFTQ
130 140 150 160 170 180
QHVPLNSNDE RIAVYGCFQN FVVVQGTRVY GWGSNTKCQL QEPKSRSLKE PVLVYDTGSV
190 200 210 220 230 240
AVDYVAMGKD FMVIVDEGGR IVHASGRLPT GFELKQQQKR HNLVVLCMWT SIHLWNARLN
250 260 270 280 290 300
TVESFGRGTH SQLFPQERLD FPIVGVATGS EHGILTTANQ EGKSHCYNVY CWGWGEHGNC
310 320 330
GPQKASQPGL QLVGQYSGKP RVFGGCATTW IVL
References and
documentation are available.
Number of amino acids: 333
Molecular weight: 36481.05
Theoretical pI: 6.25
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1596
Hydrogen H 2464
Nitrogen N 468
Oxygen O 481
Sulfur S 18
Formula: C
1596H
2464N
468O
481S
18
Total number of atoms: 5027
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 63660
Abs 0.1% (=1 g/l) 1.745, assuming all pairs of Cys residues form cystines
Ext. coefficient 62910
Abs 0.1% (=1 g/l) 1.724, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.97
This classifies the protein as stable.
Aliphatic index: 74.80
Grand average of hydropathicity (GRAVY): -0.318