ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDAEEEVVE YEEEQEEEDW SEEEEDEQEE AVEEEDGEAE PDPEGEAEAE EDKAEEVGPD 

        70         80         90        100        110        120 
EEARDAEDGP VEDSKPKPSR LFMPNLVPPK IPDGERVDFD DIHRKRMEKD LNELQTLIEA 

       130        140        150        160        170        180 
HFENRKKEEE ELISLKDRIE KRRAERAEQQ RIRNEREKER QNRLAEERAR REEEENRRKA 

       190        200        210        220        230        240 
EDEARKKKAL SNMMHFGGYI QKAQTERKSG KRQTEREKKK KILAERRKVL AIDHLNEDQL 

       250        260        270        280        290 
REKAKELWQS IHNLEAEKFD LQEKFKQQKY EINVLRNRIN DNQKVSKTRG KAKVTGRWK 

References and documentation are available.

Number of amino acids: 299

Molecular weight: 35730.28

Theoretical pI: 4.95

Amino acid composition: 
Ala (A)  22	  7.4%
Arg (R)  31	 10.4%
Asn (N)  13	  4.3%
Asp (D)  21	  7.0%
Cys (C)   0	  0.0%
Gln (Q)  15	  5.0%
Glu (E)  67	 22.4%
Gly (G)  10	  3.3%
His (H)   5	  1.7%
Ile (I)  12	  4.0%
Leu (L)  17	  5.7%
Lys (K)  34	 11.4%
Met (M)   5	  1.7%
Phe (F)   6	  2.0%
Pro (P)  10	  3.3%
Ser (S)   9	  3.0%
Thr (T)   5	  1.7%
Trp (W)   3	  1.0%
Tyr (Y)   3	  1.0%
Val (V)  11	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      1517
Hydrogen    H	      2453
Nitrogen    N	       467
Oxygen      O	       521
Sulfur      S	         5

Formula: C1517H2453N467O521S5
Total number of atoms: 4963

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20970
Abs 0.1% (=1 g/l)   0.587

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 75.28
This classifies the protein as unstable.



Aliphatic index: 55.85

Grand average of hydropathicity (GRAVY): -1.675